One-step genome editing of elite crop germplasm during haploid induction
Abstract
Genome editing using CRISPR-Cas9 works efficiently in plant cells1, but delivery of genome-editing machinery into the vast majority of crop varieties is not possible using established methods2. We co-opted the aberrant reproductive process of haploid induction (HI)3-6 to induce edits in nascent seeds of diverse monocot and dicot species. Our method, named HI-Edit, enables direct genomic modification of commercial crop varieties. HI-Edit was...
Paper Details
Title
One-step genome editing of elite crop germplasm during haploid induction
Published Date
Mar 1, 2019
Journal
Volume
37
Issue
3
Pages
287 - 292
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