Evaluating methods for Avian avulavirus-1 whole genome sequencing

Avian avulavirus-1 (AAvV-1, previously Newcastle Disease Virus) is responsible for poultry and wild birds' disease outbreaks. Numerous whole genome sequencing methods were reported for this virus. These methods included cloning, specific primers amplification, shotgun PCR approaches, Sequence Independent Single Primer Amplification and next generation sequencing platform kits.Three methods were used to sequence 173 Israeli Avian avulavirus-1...