Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract

Cell-free protein synthesis (CFPS) has become an established biotechnology tool for rapid protein expression. Over the past few decades many advances have elevated CFPS from a niche, low-efficiency system to one now capable of biomanufacturing custom proteins. Many research papers and reviews exist on the advances made in CFPS genetic template and cell extract preparation for use in E. coli systems. What is currently missing from the literature...