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Co-aggregation of ovotransferrin and lysozyme

Published on Apr 1, 2019in Food Chemistry5.40
· DOI :10.1016/j.foodhyd.2018.11.022
Kazuki Iwashita5
Estimated H-index: 5
(University of Tsukuba),
Akihiro Handa4
Estimated H-index: 4
,
Kentaro Shiraki28
Estimated H-index: 28
(University of Tsukuba)
Abstract
Abstract Ovotransferrin (OVT) is the main protein component of egg white responsible for initial gelation due to its high thermal susceptibility. Co-aggregation of lysozyme (LYZ) is involved in OVT aggregate formation at low temperatures during pasteurization. Undesirable formation of aggregates limits the degree of thermal processing that can be applied to egg white products. However, the characteristics of co-aggregates of OVT and LYZ have not been elucidated. Here, we determined the thermal co-aggregation process of OVT and LYZ in terms of protein composition, structure, intermolecular forces, and morphology. The amount of LYZ involved in co-aggregates was dependent on the amount of aggregated OVT regardless of the mixing ratio. Native LYZ had the capability of precipitating soluble OVT aggregates by non-covalent association. The co-aggregates of OVT and LYZ formed colloidal particles with a large network, which was not observed in systems consisting of either protein alone. The hierarchical co-aggregation of OVT and LYZ started from the aggregation of OVT involving LYZ non-covalently, which suppresses electrostatic repulsion between soluble OVT aggregates, followed by the growth of insoluble aggregates by disulfide bond crosslinkage between the soluble aggregates. These results provide information regarding efficient pasteurization and thermal processing of hen egg white.
  • References (39)
  • Citations (1)
References39
Newest
#1Shogo Oki (University of Tsukuba)H-Index: 2
#2Kazuki Iwashita (University of Tsukuba)H-Index: 5
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 5 authors...
#1Monique Barreto Santos (UFRRJ: Universidade Federal Rural do Rio de Janeiro)H-Index: 3
#2Angélica Ribeiro da Costa (Federal Fluminense University)H-Index: 2
Last.Edwin Elard Garcia-Rojas (Federal Fluminense University)H-Index: 7
view all 3 authors...
#1Monique Barreto Santos (UFRRJ: Universidade Federal Rural do Rio de Janeiro)H-Index: 3
#2Carlos Wanderlei Piler de Carvalho (UFRRJ: Universidade Federal Rural do Rio de Janeiro)H-Index: 14
Last.Edwin Elard Garcia-Rojas (Federal Fluminense University)H-Index: 7
view all 3 authors...
#1Taehun Hong (University of Tsukuba)H-Index: 2
#2Kazuki Iwashita (University of Tsukuba)H-Index: 5
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 4 authors...
#1Kazuki Iwashita (University of Tsukuba)H-Index: 5
#2Akihiro HandaH-Index: 4
Last.Kentaro Shiraki (University of Tsukuba)H-Index: 28
view all 3 authors...
#1Thomas Croguennec (Agrocampus Ouest)H-Index: 8
#2Guilherme M. Tavares (UFV: Universidade Federal de Viçosa)H-Index: 6
Last.Saïd Bouhallab (Agrocampus Ouest)H-Index: 6
view all 3 authors...
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