Match!

The gut microbiota promotes hepatic fatty acid desaturation and elongation in mice

Published on Dec 1, 2018in Nature Communications11.878
· DOI :10.1038/s41467-018-05767-4
Alida Kindt3
Estimated H-index: 3
(LEI: Leiden University),
Gerhard Liebisch50
Estimated H-index: 50
+ 16 AuthorsJosef Ecker15
Estimated H-index: 15
(TUM: Technische Universität München)
Abstract
Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to host physiology and metabolic diseases. We present a comprehensive multi-omics view of the effect of intestinal microbial colonization on hepatic lipid metabolism, integrating transcriptomic, proteomic, phosphoproteomic, and lipidomic analyses of liver and plasma samples from germfree and specific pathogen-free mice. Microbes induce monounsaturated fatty acid generation by stearoyl-CoA desaturase 1 and polyunsaturated fatty acid elongation by fatty acid elongase 5, leading to significant alterations in glycerophospholipid acyl-chain profiles. A composite classification score calculated from the observed alterations in fatty acid profiles in germfree mice clearly differentiates antibiotic-treated mice from untreated controls with high sensitivity. Mechanistic investigations reveal that acetate originating from gut microbial degradation of dietary fiber serves as precursor for hepatic synthesis of C16 and C18 fatty acids and their related glycerophospholipid species that are also released into the circulation.
  • References (76)
  • Citations (11)
📖 Papers frequently viewed together
58 Citations
58 Citations
296 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
References76
Newest
#1M.-S. Ducheix (University of Toulouse)H-Index: 12
#2Alexandra Montagner (University of Toulouse)H-Index: 19
Last. Hervé Guillou (University of Toulouse)H-Index: 28
view all 15 authors...
Olive oil consumption is beneficial for health as it is associated with a decreased prevalence of cancer and cardiovascular diseases. Oleic acid is, by far, the most abundant component of olive oil. Since it can be made through de novo synthesis in animals, it is not an essential fatty acid. While it has become clear that dietary oleic acid regulates many biological processes, the signaling pathway involved in these regulations remains poorly defined. In this work we tested the impact of an olei...
11 CitationsSource
#1Kelly Nicole Haas (UMass: University of Massachusetts Amherst)H-Index: 2
#2Jeffrey L. Blanchard (UMass: University of Massachusetts Amherst)H-Index: 22
An anaerobic, saccharolytic, spore-forming, butyrate-producing bacterium, strain KNHs209T, was isolated from a switchgrass microcosm seeded with forest soil. Cells were highly motile rods, often forming long filamentous chains which were easily observed moving under the microscope. Its closest phylogenetic relative was Eisenbergiella tayi (16S rRNA gene sequence identity 94.2 %), although it was easily distinguishable based on its morphology and physiology. Whole-genome sequencing enabled develo...
6 CitationsSource
#1Ilias Lagkouvardos (TUM: Technische Universität München)H-Index: 24
#2Sandra Fischer (TUM: Technische Universität München)H-Index: 5
Last. Thomas Clavel (TUM: Technische Universität München)H-Index: 34
view all 4 authors...
The importance of 16S rRNA gene amplicon profiles for understanding the influence of microbes in a variety of environments coupled with the steep reduction in sequencing costs led to a surge of microbial sequencing projects. The expanding crowd of scientists and clinicians wanting to make use of sequencing datasets can choose among a range of multipurpose software platforms, the use of which can be intimidating for non-expert users. Among available pipeline options for high-throughput 16S rRNA g...
48 CitationsSource
#1Benjamin Ruprecht (TUM: Technische Universität München)H-Index: 12
#2Jana Zecha (DKFZ: German Cancer Research Center)H-Index: 7
Last. Bernhard KusterH-Index: 64
view all 4 authors...
: Despite recent advances in mass spectrometric sequencing speed and improved sensitivity, the in-depth analysis of proteomes still widely relies on off-line peptide separation and fractionation to deal with the enormous molecular complexity of shotgun digested proteomes. While a multitude of methods has been established for off-line peptide separation using HPLC columns, their use can be limited particularly when sample quantities are scarce. In this protocol, we describe an approach which comb...
10 CitationsSource
#1Sandrine Brugiroux (LMU: Ludwig Maximilian University of Munich)H-Index: 9
#2Markus Beutler (LMU: Ludwig Maximilian University of Munich)H-Index: 5
Last. Bärbel Stecher (LMU: Ludwig Maximilian University of Munich)H-Index: 41
view all 21 authors...
Protection against enteric infections, also termed colonization resistance, results from mutualistic interactions of the host and its indigenous microbes. The gut microbiota of humans and mice is highly diverse and it is therefore challenging to assign specific properties to its individual members. Here, we have used a collection of murine bacterial strains and a modular design approach to create a minimal bacterial community that, once established in germ-free mice, provided colonization resist...
76 CitationsSource
#1Benjamin Ruprecht (TUM: Technische Universität München)H-Index: 12
#2Heiner Koch (DKFZ: German Cancer Research Center)H-Index: 6
Last. Simone Lemeer (TUM: Technische Universität München)H-Index: 29
view all 6 authors...
Phosphorylation is among the most important post-translational modifications of proteins and has numerous regulatory functions across all domains of life. However, phosphorylation is often substoichiometric, requiring selective and sensitive methods to enrich phosphorylated peptides from complex cellular digests. Various methods have been devised for this purpose and we have recently described a Fe-IMAC HPLC column chromatography setup which is capable of comprehensive, reproducible, and selecti...
4 CitationsSource
#1Benjamin Ruprecht (TUM: Technische Universität München)H-Index: 12
#2Dongxue Wang (TUM: Technische Universität München)H-Index: 4
Last. Bernhard KusterH-Index: 64
view all 6 authors...
: The bottom-up proteomic analysis of cell line and tissue samples to a depth > 10,000 proteins still represents a considerable challenge because of the sheer number of peptides generated by proteolytic digestions and the high dynamic range of protein expression. As a result, comprehensive protein coverage requires multidimensional peptide separation. Recently, off-line hydrophilic strong cation exchange (hSAX) chromatography has proven its merits for high resolution separation of peptides due t...
9 CitationsSource
#1Robert Ernst (Goethe University Frankfurt)H-Index: 17
#2Christer S. Ejsing (University of Southern Denmark)H-Index: 32
Last. Bruno Antonny (CNRS: Centre national de la recherche scientifique)H-Index: 49
view all 3 authors...
Biological membranes are complex and dynamic assemblies of lipids and proteins. Poikilothermic organisms including bacteria, fungi, reptiles, and fish do not control their body temperature and must adapt their membrane lipid composition in order to maintain membrane fluidity in the cold. This adaptive response was termed homeoviscous adaptation and has been frequently studied with a specific focus on the acyl chain composition of membrane lipids. Massspectrometry-based lipidomics can nowadays pr...
59 CitationsSource
#1Ilias LagkouvardosH-Index: 24
#2Divya JosephH-Index: 1
Last. Thomas ClavelH-Index: 34
view all 7 authors...
IMNGS: A comprehensive open resource of processed 16S rRNA microbial profiles for ecology and diversity studies
62 CitationsSource
#1Jack Bartram (UCL: University College London)H-Index: 9
#2Edward Mountjoy (UoB: University of Bristol)H-Index: 5
Last. Mike Hubank (UCL: University College London)H-Index: 31
view all 9 authors...
High-throughput sequencing (HTS) (next-generation sequencing) of the rearranged Ig and T-cell receptor genes promises to be less expensive and more sensitive than current methods of monitoring minimal residual disease (MRD) in patients with acute lymphoblastic leukemia. However, the adoption of new approaches by clinical laboratories requires careful evaluation of all potential sources of error and the development of strategies to ensure the highest accuracy. Timely and efficient clinical use of...
15 CitationsSource
Cited By11
Newest
#1Xu-Wen Wang (Brigham and Women's Hospital)
#2Yang-Yu Liu (Harvard University)
Abstract Accumulated evidence has shown that commensal microorganisms play key roles in human physiology and diseases. Dysbiosis of the human-associated microbial communities, often referred to as the human microbiome, has been associated with many diseases. Applying supervised classification analysis to the human microbiome data can help us identify subsets of microorganisms that are highly discriminative and hence build prediction models that can accurately classify unlabeled samples. Here, we...
Source
Nonalcoholic fatty liver disease (NAFLD) and obesity are becoming increasingly common globally and characteristic as gut microbiota disturbance. Supplement of probiotics is considered as a promisin...
Source
#1Matthew Wai Kin Wong (UNSW: University of New South Wales)H-Index: 5
#2Anbupalam Thalamuthu (UNSW: University of New South Wales)H-Index: 32
Last. Peter R. Schofield (NeuRA: Neuroscience Research Australia)H-Index: 93
view all 16 authors...
The critical role of blood lipids in a broad range of health and disease states is well recognised, while an understanding of the complex genetic regulation of lipid homeostasis is emerging. Traditional blood lipids (LDL-C, HDL-C and triglycerides) are known to be substantially regulated by genetic variation. Less well explored is the interplay of genetics and environment within the broader blood lipidome. Here we use the twin model to examine heritability of the plasma lipidome among healthy ol...
Source
#1Minjie ZhaoH-Index: 2
#2Zengliang JiangH-Index: 1
Last. Jing X. Kang (Harvard University)
view all 11 authors...
Obesity and associated metabolic disorders are worldwide public health issues. The gut microbiota plays a key role in the pathophysiology of diet-induced obesity. Glycerol monolaurate (GML) is a widely consumed food emulsifier with antibacterial properties. Here, we explore the anti-obesity effect of GML (1,600 mg/kg of body weight) in high-fat diet (HFD)-fed mice. HFD-fed mice were treated with 1,600 mg/kg GML. Integrated microbiome, metabolome, and transcriptome analyses were used to systemati...
Source
#1Xiaohui Li (NUPT: Nanjing University of Posts and Telecommunications)H-Index: 2
#2Yun Hu (NUPT: Nanjing University of Posts and Telecommunications)
Last. Lianhui Wang (NUPT: Nanjing University of Posts and Telecommunications)H-Index: 48
view all 9 authors...
The potential toxicity of cadmium-containing quantum dots (QDs) has received much attention because of increasing biomedical applications. However, little has been known about how cadmium telluride (CdTe) QDs influence the gut microbiota and lipid metabolism. In this study, mice were exposed orally to CdTe QDs (200 muL of 0.2, 2, 20 or 200 mum; twice per week) for 4 weeks. The oral experiments showed CdTe QD exposure led to a decrease of the Firmicutes/Bacteroidetes (F/B) ratio of gut microbiota...
Source
#1Xianglong Meng (Shanxi University)H-Index: 2
#2Junnan Ma (Dongguk University)H-Index: 1
Last. Yong-Ki Park (Dongguk University)H-Index: 23
view all 5 authors...
Background: Jowiseungki decoction (JSD) is a prescription commonly used for the treatment of diabetic complications or diabetic nephropathy (DN) in traditional medicine clinics. However, the underlying therapeutic mechanisms of JSD are still unclear. Methods: Streptozotocin (STZ)-induced DN mice were administered 100 and 500 mg/kg JSD for 4 weeks, and the therapeutic mechanisms and targets of JSD were analyzed by network pharmacology and gut microbiota analyses. Results: JSD significantly decrea...
Source
#1Xinyu Zhu (DTU: Technical University of Denmark)
#1Xinyu Zhu (DTU: Technical University of Denmark)H-Index: 8
Last. Irini Angelidaki (DTU: Technical University of Denmark)H-Index: 78
view all 8 authors...
Methanogenesis, a biological process mediated by complex microbial communities, has attracted great attention due to its contribution to global warming and potential in biotechnological applications. The current study unveiled the core microbial methanogenic metabolisms in anaerobic vessel ecosystems by applying combined genome-centric metagenomics and metatranscriptomics. Here, we demonstrate that an enriched natural system, fueled only with acetate, could support a bacteria-dominated microbiot...
2 CitationsSource
#1Robert H. MillsH-Index: 6
#2Jacob M. Wozniak (UM: University of Montana)H-Index: 5
Last. David Gonzalez (UM: University of Montana)H-Index: 28
view all 8 authors...
Connections between the microbiome and health are rapidly emerging in a wide range of diseases. However, a detailed mechanistic understanding of how different microbial communities are influencing their hosts is often lacking. One method researchers have used to understand these effects are germ-free (GF) mouse models. Differences found within the organ systems of these model organisms may highlight generalizable mechanisms that microbiome dysbioses have throughout the host. Here, we applied mul...
Source
#1I. Jane Cox (University of Cambridge)H-Index: 33
#1I. Jane Cox (University of Cambridge)
Last. Jasmohan S. Bajaj (VCU: Virginia Commonwealth University)H-Index: 54
view all 15 authors...
BACKGROUND & AIMS: Dietary changes can modulate gut microbiota and interact with cirrhosis. Our prior study demonstrated that microbial diversity was higher in cirrhotics from Turkish vs the USA, which was associated with lower risk of 90-day hospitalizations. We aimed to define gut microbial functional and metabolomic changes to increase insight into benefits of the Mediterranean compared to Western diets. METHODS: In all, 139 Turkish (46 controls/50 compensated/43 decompensated) and 157 Americ...
4 CitationsSource
#1Valentina Klaus (TUM: Technische Universität München)H-Index: 2
#2Sonja C. SchrieverH-Index: 10
Last. Jerzy AdamskiH-Index: 63
view all 16 authors...
The steadily increasing amount of newly generated omics data of various types from genomics to metabolomics is a chance and a challenge to systems biology. To fully use its potential, one key is the meaningful integration of different types of omics. We here present a fully unsupervised and versatile correlation-based method, termed Correlation guided Network Integration (CoNI), to integrate multi-omics data into a hypergraph structure that allows for identification of effective regulators. Our ...
Source