The role of β‐barrels 1 and 2 in the enzymatic activity of factor XIII A‐subunit

Essentials The roles of β-barrels 1 and 2 in factor XIII (FXIII) are currently unknown. FXIII truncations lacking β-barrel 2, both β-barrels, or full length FXIII, were made. Removing β-barrel 2 caused total loss of activity, removing both β-barrels returned 30% activity. β-barrel 2 is necessary for exposure of the active site cysteine during activation.Background Factor XIII is composed of an activation peptide segment, a β-sandwich domain, a...