Programmable activation of Bombyx gene expression using CRISPR/dCas9 fusion systems

The recently developed clustered regularly interspaced short palindromic repeats (CRISPR)‐based techniques have made it possible to reprogram target gene expression without cloning complementary DNA or disturbing genomic sequence in mammalian cells and several multicellular organisms. We previously showed that CRISPR‐associated protein 9 (Cas9) and CRISPR from Prevotella and Francisella 1 (Cpf1) could induce target mutations, deletions,...