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Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene

Published on Jul 4, 2018in British Poultry Science 1.42
· DOI :10.1080/00071668.2018.1479060
Silvana Hipatia Santander Parra5
Estimated H-index: 5
(USP: University of São Paulo),
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo)
+ 2 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
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Abstract
1. Infectious laryngotracheitis is a respiratory disease that affects the poultry industry worldwide. It is common in flocks with high-bird density, causing major economic losses.2. In this study, a SYBR® FAST polymerase chain reaction (PCR) double-strand DNA intercalating agent assay was performed for the detection of infectious laryngotracheitis virus (ILTV) in clinical samples in comparison with a conventional nested-PCR, both based on the glycoprotein E encoding gene. This assay amplified 56 bp and was capable of detecting 19 to 1 copies of virus.3. In total, 164 clinical samples were obtained from birds with respiratory problems from the period of 2009–2016. In the nested-PCR, there were 45.12% positive samples and 54.88% negative samples, while in the real-time PCR (qPCR), there were 81.1% positive samples and 18.9% negative samples.4. In conclusion, qPCR from the DNA double-strand intercalating agent SYBR® GREEN FAST was useful for the diagnosis of ILTV because it detected samples that were...
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References22
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Published on Nov 1, 2015in Journal of General Virology 2.81
Roy P1
Estimated H-index: 1
(UNE: University of New England (Australia)),
Fakhrul Islam Af1
Estimated H-index: 1
(UNE: University of New England (Australia))
+ 3 AuthorsS. W. Walkden-Brown8
Estimated H-index: 8
(UNE: University of New England (Australia))
Infectious laryngotracheitis (ILT) is an important disease of chickens caused by ILT virus (ILTV). We used the Australian SA2 and A20 vaccine strains of ILTV to determine tissue distribution and excretion characteristics of ILTV in specific-pathogen-free chickens and to determine whether ILTV is readily detectable in environmental samples such as faeces, bedding material and dust using real-time quantitative PCR. Three groups of 10 freshly hatched chicks were placed in isolators and infected ora...
2 Citations Source Cite
Published on Jun 28, 2013in PLOS ONE 2.78
Yan Zhao10
Estimated H-index: 10
(Harbin Veterinary Research Institute),
Congcong Kong3
Estimated H-index: 3
(Harbin Veterinary Research Institute)
+ 6 AuthorsYunfeng Wang18
Estimated H-index: 18
(Harbin Veterinary Research Institute)
Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no cross reactions with the DNA and RNA of infectious bursal disease virus, chicken anemia virus, reticuloe...
18 Citations Source Cite
Published on Jun 1, 2011in Avian Pathology 1.96
Alireza Mahmoudian5
Estimated H-index: 5
(University of Melbourne),
Naomi C. Kirkpatrick7
Estimated H-index: 7
(University of Melbourne)
+ 5 AuthorsAmir H. Noormohammadi24
Estimated H-index: 24
(University of Melbourne)
Infectious laryngotracheitis is an acute viral respiratory disease of chickens with a worldwide distribution. Sensitive detection of the causative herpesvirus is particularly important because it can persist in the host at a very low copy number and be transmitted to other birds. Quantification of viral genome copy number is also useful for clinical investigations and experimental studies. In the study presented here, a quantitative polymerase chain reaction (qPCR) assay was developed using SYBR...
24 Citations Source Cite
Published on Jan 1, 2009in Archives of Virology 2.26
Andrew J. Davison79
Estimated H-index: 79
(Glas.: University of Glasgow),
R. Eberle20
Estimated H-index: 20
(OSU: Oklahoma State University–Stillwater)
+ 7 AuthorsEtienne Thiry39
Estimated H-index: 39
(University of Liège)
The taxonomy of herpesviruses has been updated by the International Committee on Taxonomy of Viruses (ICTV). The former family Herpesviridae has been split into three families, which have been incorporated into the new order Herpesvirales. The revised family Herpesviridae retains the mammal, bird and reptile viruses, the new family Alloherpesviridae incorporates the fish and frog viruses, and the new family Malacoherpesviridae contains a bivalve virus. Three new genera have been created in the f...
550 Citations Source Cite
Published on Aug 1, 2008in Journal of Virological Methods 1.75
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Antonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
A standardised nested-PCR method that amplifies a region of the glycoprotein E gene of avian infectious laryngotracheitis virus (ILTV) has been developed for the diagnosis of infection by Gallid herpesvirus 1. The two sets of primers employed produced the expected amplification products of 524 bp (external primers) and 219 bp (internal primers) in the presence of ILTV DNA, whereas no such amplicons were obtained with other avian respiratory pathogens or with DNA extracted from the cells of uninf...
16 Citations Source Cite
Published on Jan 1, 2008
Dean Fraga10
Estimated H-index: 10
(College of Wooster),
Tea Meulia20
Estimated H-index: 20
(Ohio Agricultural Research and Development Center),
Steven D. Fenster9
Estimated H-index: 9
(Ashland University)
Real-time PCR is a recent modification to the polymerase chain reaction that allows precise quantification of specific nucleic acids in a complex mixture by fluorescent detection of labeled PCR products. Detection can be accomplished using specific as well as nonspecific fluorescent probes. Real-time PCR is often used in the quantification of gene expression levels. Prior to using real-time PCR to quantify a target message, care must be taken to optimize the RNA isolation, primer design, and PCR...
430 Citations Source Cite
Published on Jan 1, 2007in Journal of Virological Methods 1.75
Scott A. Callison10
Estimated H-index: 10
(UGA: University of Georgia),
Sylva M. Riblet14
Estimated H-index: 14
(UGA: University of Georgia)
+ 6 AuthorsMaricarmen García28
Estimated H-index: 28
(UGA: University of Georgia)
Abstract In this study, the development and validation of a real-time (ReTi) PCR assay is described using a Taqman ® labeled probe for the detection and quantitation of infectious larygotracheitis virus (ILTV) in chickens. The ReTi ILTV assay was highly specific with a quantitation limit of 100 viral template copies per amplification reaction. In experimentally infected, birds during early acute stages of infection, an average of 6.67 log 10 viral template copies/amplification reaction were dete...
49 Citations Source Cite
Published on Aug 15, 2006in Journal of Virology 4.32
Dean R. Thureen1
Estimated H-index: 1
,
Calvin L. Keeler1
Estimated H-index: 1
Psittacid herpesvirus 1 (PsHV-1) is the causative agent of Pacheco's disease, an acute, highly contagious, and potentially lethal respiratory herpesvirus infection in psittacine birds, while infectious laryngotracheitis virus (ILTV) is a highly contagious and economically significant avian herpesvirus which is responsible for an acute respiratory disease limited to galliform birds. The complete genome sequence of PsHV-1 has been determined and compared to the ILTV sequence, assembled from publis...
62 Citations Source Cite
Published on Apr 1, 2006in Avian Pathology 1.96
Julie L. Creelan12
Estimated H-index: 12
,
V. Calvert10
Estimated H-index: 10
+ 1 AuthorsSamuel J. McCullough4
Estimated H-index: 4
A real-time polymerase chain reaction (PCR) assay was developed to specifically amplify infectious laryngotracheitis virus (ILTV) DNA from field samples. The 222-base-pair PCR fragment was amplified using primers located in a conserved region of the infected cell protein 4 gene that was demonstrated in this work to encompass a single nucleotide polymorphism. Subsequent restriction fragment length polymorphism (RFLP) analysis of real-time PCR amplified fragments from a range of ILTV isolates usin...
49 Citations Source Cite
Published on Apr 1, 2004in Avian Diseases 1.31
Holly S. Sellers16
Estimated H-index: 16
,
Maricarmen García28
Estimated H-index: 28
+ 3 AuthorsJames S. Guy26
Estimated H-index: 26
Abstract During 2001, a mild infectious laryngotracheitis virus (ILTV) infection occurred in broiler flocks in the southeastern United States. Clinical signs included mild tracheitis, swollen sinuses, and conjunctivitis, with no increased mortality and minimal serologic response. Infrequent intranuclear inclusion bodies with or without syncytial cell formation were observed in eyelid, trachea, and larynx and in the chorioallantoic membrane of infected embryos. Immunohistochemistry and a nested i...
28 Citations Source Cite
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