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Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene

Published on Jul 4, 2018in British Poultry Science1.42
· DOI :10.1080/00071668.2018.1479060
Silvana H. Santander Parra5
Estimated H-index: 5
(USP: University of São Paulo),
Luis Fabian N Nuñez5
Estimated H-index: 5
(USP: University of São Paulo)
+ 2 AuthorsAntonio José Piantino Ferreira24
Estimated H-index: 24
(USP: University of São Paulo)
Abstract
1. Infectious laryngotracheitis is a respiratory disease that affects the poultry industry worldwide. It is common in flocks with high-bird density, causing major economic losses.2. In this study, a SYBR® FAST polymerase chain reaction (PCR) double-strand DNA intercalating agent assay was performed for the detection of infectious laryngotracheitis virus (ILTV) in clinical samples in comparison with a conventional nested-PCR, both based on the glycoprotein E encoding gene. This assay amplified 56 bp and was capable of detecting 19 to 1 copies of virus.3. In total, 164 clinical samples were obtained from birds with respiratory problems from the period of 2009–2016. In the nested-PCR, there were 45.12% positive samples and 54.88% negative samples, while in the real-time PCR (qPCR), there were 81.1% positive samples and 18.9% negative samples.4. In conclusion, qPCR from the DNA double-strand intercalating agent SYBR® GREEN FAST was useful for the diagnosis of ILTV because it detected samples that were...
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