A Red Fluorescent Protein for Cryogenic Single-Molecule Superresolution Imaging

Superresolution imaging techniques routinely resolve fluorescently labelled structures in cells with resolution more than an order of magnitude beyond the diffraction limit. The molecular specificity and spatial resolution of these techniques make them a powerful tool for the life sciences. In single-molecule superresolution imaging, the precision with which a single molecule can be localized depends on the number of photons collected before...