Original paper

A highly specific SpCas9 variant is identified by in vivo screening in yeast

Volume: 36, Issue: 3, Pages: 265 - 271
Published: Jan 29, 2018
Abstract
Despite the utility of CRISPR-Cas9 nucleases for genome editing, the potential for off-target activity limits their application, especially for therapeutic purposes. We developed a yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous evaluation of on- and off-target activity. We screened a library of SpCas9 variants carrying random mutations in the REC3 domain and identified mutations...
Paper Details
Title
A highly specific SpCas9 variant is identified by in vivo screening in yeast
Published Date
Jan 29, 2018
Volume
36
Issue
3
Pages
265 - 271
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