Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
Abstract
Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in Escherichia coli, the expression and solubility of hFGF21 were tested and optimised by fusing the protein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubiquitin-related modifier (Sumo), glutathione S-transferase (GST), maltose-binding protein...
Paper Details
Title
Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
Published Date
Nov 23, 2017
Journal
Volume
7
Issue
1
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