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Comparative transcriptome analysis of Apis mellifera antennae of workers performing different tasks

Published on Feb 1, 2018in Molecular Genetics and Genomics2.88
· DOI :10.1007/s00438-017-1382-5
Hongyi Nie3
Estimated H-index: 3
(Fujian Agriculture and Forestry University),
Shupeng Xu1
Estimated H-index: 1
(Fujian Agriculture and Forestry University)
+ 7 AuthorsSongkun Su3
Estimated H-index: 3
(Fujian Agriculture and Forestry University)
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Abstract
Honey bee is a social insect. Its colony is mainly coordinated by the chemical signals such as pheromones produced by queen or brood. Correspondingly, the worker bee developed numerous complicated olfactory sensilla in antennae for detection of these colony chemical signals and nectar/pollen signals in foraging. With the normal development of new emerged workers, young adults (nurse bee) worked in colony at the first 2–3 weeks and then followed by the foraging activity outside of the hive, which give rise to great change of the surrounding chemical signals. However, the olfactory adaption mechanism of worker bee in these processes of behavioral development is still unclear. In this study, we conducted a comprehensive and quantitative analysis of gene expression in Apis mellifera antenna of newly emerged workers, nurses and foragers using transcriptome analysis. Meanwhile, we constructed experimental colonies to collect age-matched samples, which were used to determine whether task is the principal determinant of differential expression. RNA sequencing and quantitative real-time polymerase chain reaction revealed that 6 and 14 genes were closely associated with nurse and forager behaviors, respectively. Furthermore, a broad dynamic range of chemosensory gene families and candidate odorant degrading enzymes were analyzed at different behavior statuses. We firstly reported genes associated with nursing/foraging behavior from antennae and the variations of expression of genes belonging to various olfactory gene families at different development stages. These results not only could contribute to elucidating the relationship between olfactory and behavior-related changes, but also provide a new perspective into the molecular mechanism underlying honey bee division of labor.
  • References (66)
  • Citations (4)
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References66
Newest
Published on Feb 11, 2018in Veterinarni Medicina0.64
M. Trhlin1
Estimated H-index: 1
,
J. Rajchard1
Estimated H-index: 1
An important area of physiology of the honeybee ( Apis mellifera) is chemical communication between individuals and castes in the swarm, which maintains its integrity and function. The highly complex social organiza - tion of honeybees is mediated through pheromones. Releaser pheromones cause rapid changes in the behaviour of the recipient, while primer pheromones have relatively slow and long-term effects on the physiology and behaviour of the recipient. Queen retinue pheromone (QRP) is a blend...
Published on May 1, 2017in Scientific Reports4.01
Faisal Younus1
Estimated H-index: 1
(CSIRO: Commonwealth Scientific and Industrial Research Organisation),
Nicholas J. Fraser5
Estimated H-index: 5
+ 7 AuthorsJohn G. Oakeshott48
Estimated H-index: 48
(CSIRO: Commonwealth Scientific and Industrial Research Organisation)
Previous electrophysiological and behavioural studies implicate esterase 6 in the processing of the pheromone cis-vaccenyl acetate and various food odorants that affect aggregation and reproductive behaviours. Here we show esterase 6 has relatively high activity against many of the short-mid chain food esters, but negligible activity against cis-vaccenyl acetate. The crystal structure of esterase 6 confirms its substrate-binding site can accommodate many short-mid chain food esters but not cis-v...
Published on Sep 1, 2016in Scientific Reports4.01
Anton Stabentheiner20
Estimated H-index: 20
,
Helmut Kovac14
Estimated H-index: 14
Economic principles play an important role not only in human but also in animal communities1,2,3,4,5. The application of economic principles by animals implies finding a positive balance between energetic costs and gains, or between investment and returns2,3. Among the insects, foragers like honeybees are of special interest because they combine high energetic costs with high gains in a widely and wildly fluctuating environment. The high costs result from endothermy kept up throughout the foragi...
Published on Apr 1, 2016in Scientific Reports4.01
Xu Jiang He4
Estimated H-index: 4
(JXAU: Jiangxi Agricultural University),
Xue Chuan Zhang1
Estimated H-index: 1
+ 3 AuthorsZhi Jiang Zeng7
Estimated H-index: 7
(JXAU: Jiangxi Agricultural University)
Communication between parents and young offspring for food provisioning presents an area of potential conflict between the amount of food requested by young and the optimal resource allocation by the parents. This has become an area of increasing interest to evolutionary biologists1. Recent studies mainly focused on parent-offspring conflicts in mammals and avian species2,3,4. In these species since parents are equally related to their offspring they are expected to favour an even division of fo...
Published on Dec 1, 2015in Genome Biology14.03
Vihandha O. Wickramasinghe10
Estimated H-index: 10
(Medical Research Council),
Mar Gonzàlez-Porta10
Estimated H-index: 10
(EMBL-EBI: European Bioinformatics Institute)
+ 6 AuthorsAshok R. Venkitaraman49
Estimated H-index: 49
(Medical Research Council)
Background Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. The core spliceosome component PRPF8 is essential for spliceosome assembly through its participation in ribonucleoprotein (RNP) complexes for splice-site recognition, branch-point formation and catalysis. PRPF8 deficiency is linked to human diseases like retinitis pigmentosa or myeloid neoplasia, but its genome-wide effects on constitutive and alternative splicing remain ...
Published on Nov 20, 2015in PLOS ONE2.78
Yisilahaiti Paerhati1
Estimated H-index: 1
(Iwate University),
Shinichi Ishiguro1
Estimated H-index: 1
(Iwate University)
+ 6 AuthorsKoichi Suzuki13
Estimated H-index: 13
(Iwate University)
We investigated the association between the expression of a gene encoding gustatory receptor (G10) and division of labor in the honey bee, Apis mellifera. Among 10 GR genes encoding proteins 15% ~ 99% amino acid identity in the honey bee, we found that AmGR10 with 99% identity is involved in nursing or brood care. Expression of AmGR10 was restricted to organs of the hypopharyngeal gland, brain, and ovary in the nurse bee phase. Members of an extended nursing caste under natural conditions contin...
Published on Nov 5, 2015in Frontiers in Physiology3.20
Thomas Chertemps6
Estimated H-index: 6
(University of Paris),
Faisal Younus3
Estimated H-index: 3
(ANU: Australian National University)
+ 5 AuthorsMartine Maïbèche4
Estimated H-index: 4
(University of Paris)
Reception of odorant molecules within insect olfactory organs involves several sequential steps, including their transport through the sensillar lymph, interaction with the respective sensory receptors, and subsequent inactivation. Odorant-degrading enzymes (ODEs) putatively play a role in signal dynamics by rapid degradation of odorants in the vicinity of the receptors, but this hypothesis is mainly supported by in vitro results. We have recently shown that an extracellular carboxylesterase, es...
Published on Oct 1, 2015in Scientific Reports4.01
Masaru Hojo23
Estimated H-index: 23
,
Kenichi Ishii11
Estimated H-index: 11
+ 3 AuthorsMamiko Ozaki17
Estimated H-index: 17
Chemical communication is essential for the coordination of complex organisation in ant societies. Recent comparative genomic approaches have revealed that chemosensory genes are diversified in ant lineages, and suggest that this diversification is crucial for social organisation. However, how such diversified genes shape the peripheral chemosensory systems remains unknown. In this study, we annotated and analysed the gene expression profiles of chemosensory proteins (CSPs), which transport lipo...
Published on Oct 1, 2015in Insect Biochemistry and Molecular Biology3.62
Sylvain Forêt25
Estimated H-index: 25
(ANU: Australian National University),
Kevin W. Wanner16
Estimated H-index: 16
(UIUC: University of Illinois at Urbana–Champaign),
Ryszard Maleszka43
Estimated H-index: 43
(ANU: Australian National University)
Published on Oct 1, 2015in Insect Molecular Biology2.44
Wenfu Mao13
Estimated H-index: 13
(UIUC: University of Illinois at Urbana–Champaign),
Mary A. Schuler54
Estimated H-index: 54
(UIUC: University of Illinois at Urbana–Champaign),
May R. Berenbaum67
Estimated H-index: 67
(UIUC: University of Illinois at Urbana–Champaign)
In insects, cytochrome P450 monooxygenases (P450s) contribute to phytochemical and pheromone clearance in chemoreception and xenobiotic detoxification in food processing. In eusocial species, P450 expression varies with anatomy and age-related behaviour. Adult honeybees (Apis mellifera) possess appendages differentially equipped for chemoreception; antennae and prothoracic and mesothoracic legs assess food and pheromone signals whereas metathoracic legs transport pollen over long distances. Newl...
Cited By4
Newest
Published on 2018in Chronobiology International2.56
Rikesh Jain2
Estimated H-index: 2
(NCBS: National Centre for Biological Sciences),
Axel Brockmann13
Estimated H-index: 13
(NCBS: National Centre for Biological Sciences)
ABSTRACTHoney bees have a remarkable sense of time and individual honey bee foragers are capable of adjusting their foraging activity with respect to the time of food availability. Although, there is compelling experimental evidence that foraging behavior is guided by the circadian clock, nothing is known about the underlying molecular mechanisms. Here we present for the first time a study that explores whether time-restricted foraging under natural light-dark (LD) condition affects the molecula...
Published on Oct 1, 2018in Insect Molecular Biology2.44
Bernarda Calla11
Estimated H-index: 11
(UIUC: University of Illinois at Urbana–Champaign),
Marina MacLean3
Estimated H-index: 3
(UNR: University of Nevada, Reno)
+ 4 AuthorsMay R. Berenbaum67
Estimated H-index: 67
(UIUC: University of Illinois at Urbana–Champaign)
Published on Jun 20, 2018in Frontiers in Physiology3.20
Immacolata Iovinella13
Estimated H-index: 13
(UniFI: University of Florence),
Federico Cappa7
Estimated H-index: 7
(UniFI: University of Florence)
+ 4 AuthorsFrancesca Romana Dani28
Estimated H-index: 28
(UniFI: University of Florence)
Reproductive and task partitioning in large colonies of social insects suggest that colony members belonging to different castes or performing different tasks during their life (polyethism) may produce specific semiochemicals and be differently sensitive to the variety of pheromones involved in intraspecific chemical communication. The main peripheral olfactory organs are the antennal chemosensilla, where the early olfactory processes take place. At this stage, members of two different families ...
Published on Jan 1, 2018in Comparative and Functional Genomics2.30
Hongyi Nie3
Estimated H-index: 3
(Fujian Agriculture and Forestry University),
Haiyang Geng2
Estimated H-index: 2
(Fujian Agriculture and Forestry University)
+ 4 AuthorsSongkun Su3
Estimated H-index: 3
(Fujian Agriculture and Forestry University)
The forkhead box (Fox) gene family, one of the most important families of transcription factors, participates in various biological processes. However, Fox genes in Hymenoptera are still poorly known. In this study, 14 Fox genes were identified in the genome of Apis cerana. In addition, 16 (Apis mellifera), 13 (Apis dorsata), 16 (Apis florea), 17 (Bombus terrestris), 16 (Bombus impatiens), and 18 (Megachile rotundata) Fox genes were identified in their genomes, respectively. Phylogenetic analyse...
View next paperHoney bee (Apis mellifera) larval pheromones regulate gene expression related to foraging task specialization