CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles

Significance Using budding yeast, we address how Cas9 protein and its guide RNA (gRNA) create double-strand chromosome breaks (DSBs), and explore whether binding of Cas9::gRNA influences subsequent DSB repair by nonhomologous end-joining. We created pairs of gRNAs that are complementary to opposite DNA strands but direct cleavage at the same chromosomal location. The resulting repair profiles (insertion/deletions) are different for the two...