An immobilized and highly stabilized self-sufficient monooxygenase as biocatalyst for oxidative biotransformations

BACKGROUND The requirement for expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self‐sufficient Baeyer–Villiger monooxygenases is discussed. RESULTS A bifunctional biocatalyst composed of an NADPH‐dependent cyclohexanone monooxygenase (CHMO) fused to an NADP + ‐accepting...