Classical autophagy proteins LC3B and ATG4B facilitate melanosome movement on cytoskeletal tracks

Published on Aug 3, 2017in Autophagy11.06
· DOI :10.1080/15548627.2017.1327509
Amrita Ramkumar3
Estimated H-index: 3
(Institute of Genomics and Integrative Biology),
Divya Murthy6
Estimated H-index: 6
(Institute of Genomics and Integrative Biology)
+ 10 AuthorsRajesh S. Gokhale31
Estimated H-index: 31
(Institute of Genomics and Integrative Biology)
ABSTRACTMacroautophagy/autophagy is a dynamic and inducible catabolic process that responds to a variety of hormonal and environmental cues. Recent studies highlight the interplay of this central pathway in a variety of pathophysiological diseases. Although defective autophagy is implicated in melanocyte proliferation and pigmentary disorders, the mechanistic relationship between the 2 pathways has not been elucidated. In this study, we show that autophagic proteins LC3B and ATG4B mediate melanosome trafficking on cytoskeletal tracks. While studying melanogenesis, we observed spatial segregation of LC3B-labeled melanosomes with preferential absence at the dendritic ends of melanocytes. This LC3B labeling of melanosomes did not impact the steady-state levels of these organelles but instead facilitated their intracellular positioning. Melanosomes primarily traverse on microtubule and actin cytoskeletal tracks and our studies reveal that LC3B enables the assembly of microtubule translocon complex. At the mic...
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