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MicroRNAs in Palatogenesis and Cleft Palate

Published on Apr 4, 2017in Frontiers in Physiology3.201
路 DOI :10.3389/fphys.2017.00165
Christian Schoen1
Estimated H-index: 1
(Radboud University Nijmegen),
Armaz Aschrafi25
Estimated H-index: 25
(LMB: Laboratory of Molecular Biology)
+ 3 AuthorsCarine Carels29
Estimated H-index: 29
(Katholieke Universiteit Leuven)
Abstract
Palatogenesis requires a precise spatiotemporal regulation of gene expression, which is controlled by an intricate network of transcription factors and their corresponding DNA motifs. Even minor perturbations of this network may cause cleft palate, the most common congenital craniofacial defect in humans. MicroRNAs (miRNAs), a class of small regulatory non-coding RNAs, have elicited strong interest as key regulators of embryological development, and as etiological factors in disease. MiRNAs function as post-transcriptional repressors of gene expression and are therefore able to fine-tune gene regulatory networks. Several miRNAs are already identified to be involved in congenital diseases. Recent evidence from research in zebrafish and mice indicates that miRNAs are key factors in both normal palatogenesis and cleft palate formation. Here, we provide an overview of recently identified molecular mechanisms underlying palatogenesis involving specific miRNAs, and discuss how dysregulation of these miRNAs may result in cleft palate.
  • References (77)
  • Citations (9)
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References77
Newest
#1Jingyun LiH-Index: 7
#2Jijun ZouH-Index: 2
Last. Jun LiH-Index: 7
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// Jingyun Li 1, * , Jijun Zou 2, * , Qian Li 1 , Ling Chen 1 , Yanli Gao 1 , Hui Yan 1 , Bei Zhou 1 , Jun Li 1 1 State Key Laboratory of Reproductive Medicine, Department of Plastic and Cosmetic Surgery, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China 2 Department of Burns and Plastic Surgery, Children鈥檚 Hospital of Nanjing Medical University, Nanjing 210008, China * These authors contributed equally...
11 CitationsSource
#1Terri H. BeatyH-Index: 65
#2Mary L. Marazita (University of Pittsburgh)H-Index: 55
Last. Elizabeth J. Leslie (University of Pittsburgh)H-Index: 21
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Orofacial clefts include cleft lip (CL), cleft palate (CP), and cleft lip and palate (CLP), which combined represent the largest group of craniofacial malformations in humans with an overall prevalence of one per 1,000 live births. Each of these birth defects shows strong familial aggregation, suggesting a major genetic component to their etiology. Genetic studies of orofacial clefts extend back centuries, but it has proven difficult to define any single etiologic mechanism because many genes ap...
44 CitationsSource
#1Dandan LiH-Index: 6
#2Hongchuang ZhangH-Index: 4
Last. Yongchu PanH-Index: 12
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We hypothesized that microRNA binding site single nucleotide polymorphisms (SNPs) in fibroblast growth factors (FGFs) and their receptor genes (FGFRs) may affect microRNA and mRNA interactions and are thereby associated with susceptibility of non-syndromic orofacial cleft (NSOC). Ten SNPs among the FGF and FGFR genes were selected and their associations with NSOC susceptibility were investigated in a case-control study of 602 patients with NSOC and 605 healthy controls. FGF2/rs1048201, FGF5/rs37...
5 CitationsSource
#1Jijun Zou (Nanjing Medical University)H-Index: 2
#2Jingyun Li (Nanjing Medical University)H-Index: 2
Last. Xi-rongGUO (Nanjing Medical University)H-Index: 27
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Abstract Objective The aim of this study was to determine the expression profile of plasma microRNAs in nonsyndromic cleft lip (NSCL) and their clinical significance as biomarkers. Methods Agilent human miRNA microarray chips were used to analyze three NSCL plasma samples (mixed as CL group) and three normal plasma samples (mixed as Control group). Six selected plasma miRNAs were validated using qRT-PCR between another 13 CL and 11 healthy children. The receiver operating characteristic (ROC) cu...
5 CitationsSource
#1Hai-Lei Ding (Anschutz Medical Campus)H-Index: 2
#2Joan E. Hooper (Anschutz Medical Campus)H-Index: 19
Last. David E. Clouthier (Anschutz Medical Campus)H-Index: 27
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Defects in mid-facial development, including cleft lip/palate, account for a large number of human birth defects annually. In many cases, aberrant gene expression results in either a reduction in the number of neural crest cells (NCCs) that reach the frontonasal region and form much of the facial skeleton or subsequent failure of NCC patterning and differentiation into bone and cartilage. While loss of gene expression is often associated with developmental defects, aberrant upregulation of expre...
13 CitationsSource
#1Shan Gao (A&M: Texas A&M University)H-Index: 5
#2Myriam Moreno (UI: University of Iowa)H-Index: 4
Last. Brad A. Atnendt (UI: University of Iowa)H-Index: 33
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T-box transcription factor TBX1 is the major candidate gene for 22q11.2 deletion syndrome (22q11.2DS, DiGeorge syndrome/ Velo-cardio-facial syndrome), whose phenotypes include craniofacial malformations such as dental defects and cleft palate. In this study, Tbx1 was conditionally deleted or over-expressed in the oral and dental epithelium to establish its role in odontogenesis and craniofacial developmental. Tbx1 lineage tracing experiments demonstrated a specific region of Tbx1positive cells i...
21 CitationsSource
#1Ratnam S. SeelanH-Index: 10
#2Partha MukhopadhyayH-Index: 15
Last. Robert M. GreeneH-Index: 22
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Environmental factors contribute to the etiology of cleft palate (CP). Environmental factors can also affect gene expression via alterations in DNA methylation suggesting a possible mechanism for the induction of CP. Identification of genes methylated during development of the secondary palate provides the basis for examination of the means by which environmental factors may adversely influence palatal ontogeny. We previously characterized the methylome of the developing murine secondary palate ...
2 CitationsSource
#1Dennis R. Warner (University of Louisville)H-Index: 14
#2Partha Mukhopadhyay (University of Louisville)H-Index: 15
Last. Robert M. Greene (University of Louisville)H-Index: 22
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Clefts of the lip and palate are thought to be caused by genetic and environmental insults but the role of epigenetic mechanisms underlying this common birth defect are unknown. We analyzed the expression of over 600 microRNAs in the murine medial nasal and maxillary processes isolated on GD10.0鈥揋D11.5 to identify those expressed during development of the upper lip and analyzed spatial expression of a subset. A total of 142 microRNAs were differentially expressed across gestation days 10.0鈥11.5 ...
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#1Lan Ma (Nanjing Medical University)H-Index: 15
#2Min Xu (Nanjing Medical University)H-Index: 4
Last. Lin Wang (Nanjing Medical University)H-Index: 20
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MSX1 is a favorable candidate gene for susceptibility to non-syndromic orofacial clefts (NSOCs). However, the roles of MSX1 genetic variants in the development of NSOC are controversial and vary among human populations. In the present study, the roles of 4 potentially functional single-nucleotide polymorphisms (SNPs) of MSX1 (rs12532 in 3鈥-untranslated region [UTR], and rs3821947, rs3821949, and rs4464513 in 5鈥 upstream) were investigated in a case-control study of 602 NSOC cases and 605 healthy...
20 CitationsSource
#1Mingliang Zhou (Sichuan University)H-Index: 5
#2Junrong Ma (Sichuan University)H-Index: 7
Last. Xijie Yu (Sichuan University)H-Index: 20
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MicroRNAs (miRNAs) have been identified to play important functions during osteoblast proliferation, differentiation, and apoptosis. The miR-17~92 cluster is highly conserved in all vertebrates. Loss-of-function of the miR-17-92 cluster results in smaller embryos and immediate postnatal death of all animals. Germline hemizygous deletions of MIR17HG are accounted for microcephaly, short stature, and digital abnormalities in a few cases of Feingold syndrome. These reports indicate that miR-17~92 m...
42 CitationsSource
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#1Sissy Maria dos Anjos Mendes (UFPA: Federal University of Par谩)
#2Daybelis Del Socorro Gonz谩lez Espinosa (UFPA: Federal University of Par谩)
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#1Wancong Zhang (STU: Shantou University)
#2Zhiwei Shen (STU: Shantou University)H-Index: 6
Last. Shijie Tang (STU: Shantou University)H-Index: 7
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Abstract Background The molecular mechanisms of abnormal palatogenesis were investigated in this study. A key regulator, miR-106a-5p, and its target pathway were analyzed. Objectives This research is trying to clarify the underlying mechanism of the modulation of miRNA transcription during the formation of cleft palate by 7T and 9.4T NMR metabolomic platforms. Method Differentially expressed miRNAs and mRNAs were analyzed by microarray analysis and verified by qRT-PCR. The protein expression in ...
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#1Santiago Cerrizuela (CONICET: National Scientific and Technical Research Council)H-Index: 3
#2Guillermo A. Vega-Lopez (CONICET: National Scientific and Technical Research Council)H-Index: 3
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#1Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 20
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Fish larvae differ greatly from the adult form in their morphology and organ functionality. The functionality of the gastrointestinal tract depends on the expression of various pumps, transporters and channels responsible for feed digestion and nutrients absorption. During the larval period, the gastrointestinal tract develops from a simple closed tube, into its complex form with differentiated segments, crypts and villi, as found in the adult. In this study, we characterized the expression of t...
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The rise in atmospheric CO2 concentrations has profound impact on nutritional, metabolic and physiological activities of crop plants. In this study, the impact of elevated CO2 ranging from 350 to 4000 ppm on l-ascorbic acid (AsA) content, antioxidative properties and growth characteristics of four cruciferous vegetable seedlings (Chinese cabbage, bok choy, radish and red young radish) was analyzed. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and high performance liquid...
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Last. Belen TornesiH-Index: 10
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Abstract Predicting embryotoxicity of pharmaceutical compounds or industrial chemicals is crucial for public safety. Conventional studies which monitor embryo鈥揻etal development in rats and rabbits are costly and time consuming. Alternative assays which are simpler and less costly are being pursued. The purpose of this research was to assess the capacity for the zebrafish development assay to predict mammalian plasma levels that are embryotoxic. Previously published data on rat plasma levels asso...
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#1Mona Gajera (University of Texas Health Science Center at Houston)H-Index: 1
#2Neha Desai (University of Texas Health Science Center at Houston)
Last. Junichi Iwata (University of Texas Health Science Center at Houston)H-Index: 21
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Background The etiology of cleft lip with or without cleft palate (CL/P), a common congenital birth defect, is complex and involves the contribution of genetic and environmental factors. Although many candidate genes have been identified, the regulation and interaction of these genes in CL/P remain unclear. In addition, the contribution of microRNAs (miRNAs), non-coding RNAs that regulate the expression of multiple genes, to the etiology of CL/P is largely unknown.
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#1Soo Kyung Nam (IIT: Inha University)H-Index: 1
#2Juyoung Lee (IIT: Inha University)H-Index: 10
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Abstract Background Cleft lip with or without cleft palate (CL/P) is one of the most common congenital defects, which etiology involves both genetic and environmental factors. Previous studies have shown that miR-199a-5p may mediate the occurrence of CL/P. However, the key target genes regulated by miR-199a-5p are not clear. In this study, we employed a systematic bioinformatics analysis of target genes regulated by miR-199a-5p which may be involved in CL/P. Methods The miRBase, Human miRNA tiss...
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