Single-stranded DNA library preparation from highly degraded DNA usingT4DNA ligase
Abstract
DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more efficient if each of the two DNA strands are converted into library molecules separately. We present a new method for single-stranded library preparation, ssDNA2.0, which is based on single-stranded DNA...
Paper Details
Title
Single-stranded DNA library preparation from highly degraded DNA usingT4DNA ligase
Published Date
Jan 23, 2017
Journal
Pages
gkx033 - gkx033
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