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Influence of Knee Immobilization on Chondrocyte Apoptosis and Histological Features of the Anterior Cruciate Ligament Insertion and Articular Cartilage in Rabbits

Published on Jan 26, 2017in International Journal of Molecular Sciences4.183
· DOI :10.3390/ijms18020253
Hirotaka Mutsuzaki12
Estimated H-index: 12
,
Hiromi Nakajima8
Estimated H-index: 8
+ 2 AuthorsMasataka Sakane26
Estimated H-index: 26
Abstract
This study examined the influence of immobilization on chondrocyte apoptosis and histological features of the anterior cruciate ligament (ACL) insertion and knee articular cartilage in rabbits. Forty-eight male Japanese white rabbits were assigned to an immobilization (n = 24) or sham (n = 24) group. Rabbits in the immobilization group underwent complete unilateral surgical knee immobilization and rabbits in the sham group underwent a sham surgery. The average thickness of the glycosaminoglycan (GAG) stained red area by safranin O staining, the chondrocyte apoptosis rate and the chondrocyte proliferation rate in the cartilage layer in the ACL insertion and the articular cartilage of the medial tibial condyle were measured at one, two, four and eight weeks in six animals from each group. In the ACL insertion, the chondrocyte apoptosis rate was higher in the immobilization group than in the sham group at two and eight weeks after surgery (p < 0.05). The chondrocyte proliferation rate gradually decreased from two weeks to eight weeks in the immobilization group. The GAG layer was thinner in the immobilization group than in the sham group at two, four and eight weeks after surgery (p < 0.05). In the articular cartilage, the chondrocyte apoptosis rate in the immobilization group was higher than in the sham group at four and eight weeks after surgery (p < 0.05). The GAG layer was significantly thinner in the immobilization group than that in the sham group at four and eight weeks after surgery (p < 0.05). Knee immobilization significantly increased chondrocyte apoptosis at two and eight weeks after surgery in the ACL insertion and at four and eight weeks after surgery in the articular cartilage of the medial tibial condyle, and decreased GAG layer thickness from two to eight weeks after surgery in the ACL insertion and from four to eight weeks after surgery in the articular cartilage.
  • References (18)
  • Citations (4)
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References18
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#1Hirotaka Mutsuzaki (Ibaraki Prefectural University of Health Sciences)H-Index: 12
#2Hiromi Nakajima (Ibaraki University)H-Index: 8
Last. Masataka Sakane (University of Tsukuba)H-Index: 26
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Abstract Background The purpose of this study was to clarify the influence of mechanical unloading on histological changes of the patellar tendon (PT) insertion in rabbits. Materials and methods The PT was completely released from stress by drawing the patella toward the tibial tubercle with a stainless steel wire installed between the patella and tibial tubercle (mechanical unloading group, n=28). The animals of the sham group underwent the same surgical procedure; however, the wire was not tig...
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#1Diogo Correa Maldonado (UNIFESP: Federal University of São Paulo)H-Index: 2
#2Marcelo Cavenaghi Pereira da Silva (UNIFESP: Federal University of São Paulo)H-Index: 11
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Studies have determined the effects of joint immobilization on the articular cartilage of sedentary animals, but we are not aware of any studies reporting the effects of joint immobilization in previously trained animals. The objective of the present study was to determine whether exercise could prevent degeneration of the articular cartilage that accompanies joint immobilization. We used light microscopy to study the thickness, cell density, nuclear size, and collagen density of articular carti...
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#1Masataka Sakane (University of Tsukuba)H-Index: 26
#2Hirotaka Mutsuzaki (Ibaraki Prefectural University of Health Sciences)H-Index: 12
Last. Naoyuki Ochiai (University of Tsukuba)H-Index: 31
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Purpose The purpose of this study was to clarify the effects of partial resection on the glycosaminoglycan (GAG) layer thicknesses and chondrocyte turnover (apoptosis and cell proliferation) between uncalcified fibrocartilage (UF) and calcified fibrocartilage (CF) layers in an anterior cruciate ligament (ACL) insertion.
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#1Hirotaka Mutsuzaki (Ibaraki Prefectural University of Health Sciences)H-Index: 12
#2Masataka Sakane (University of Tsukuba)H-Index: 26
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Last. Naoyuki Ochiai (University of Tsukuba)H-Index: 31
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We examined time-dependent histological changes of the calcified fibrocartilage area in a tibial cranial cruciate ligament (CCL) insertion after ligament resection in rabbits. The animals were divided into two groups: those undergoing CCL substance resection in the right stifle (resected group) and those receiving the same operation without CCL resection in the left stifle (sham operated group). Five animals were euthanized with deep anaesthesia at four time periods (1, 2, 4 and 6 weeks), and Ha...
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