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Influence of Knee Immobilization on Chondrocyte Apoptosis and Histological Features of the Anterior Cruciate Ligament Insertion and Articular Cartilage in Rabbits

Published on Jan 26, 2017in International Journal of Molecular Sciences4.18
· DOI :10.3390/ijms18020253
Hirotaka Mutsuzaki11
Estimated H-index: 11
,
Hiromi Nakajima8
Estimated H-index: 8
+ 2 AuthorsMasataka Sakane26
Estimated H-index: 26
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Abstract
This study examined the influence of immobilization on chondrocyte apoptosis and histological features of the anterior cruciate ligament (ACL) insertion and knee articular cartilage in rabbits. Forty-eight male Japanese white rabbits were assigned to an immobilization (n = 24) or sham (n = 24) group. Rabbits in the immobilization group underwent complete unilateral surgical knee immobilization and rabbits in the sham group underwent a sham surgery. The average thickness of the glycosaminoglycan (GAG) stained red area by safranin O staining, the chondrocyte apoptosis rate and the chondrocyte proliferation rate in the cartilage layer in the ACL insertion and the articular cartilage of the medial tibial condyle were measured at one, two, four and eight weeks in six animals from each group. In the ACL insertion, the chondrocyte apoptosis rate was higher in the immobilization group than in the sham group at two and eight weeks after surgery (p < 0.05). The chondrocyte proliferation rate gradually decreased from two weeks to eight weeks in the immobilization group. The GAG layer was thinner in the immobilization group than in the sham group at two, four and eight weeks after surgery (p < 0.05). In the articular cartilage, the chondrocyte apoptosis rate in the immobilization group was higher than in the sham group at four and eight weeks after surgery (p < 0.05). The GAG layer was significantly thinner in the immobilization group than that in the sham group at four and eight weeks after surgery (p < 0.05). Knee immobilization significantly increased chondrocyte apoptosis at two and eight weeks after surgery in the ACL insertion and at four and eight weeks after surgery in the articular cartilage of the medial tibial condyle, and decreased GAG layer thickness from two to eight weeks after surgery in the ACL insertion and from four to eight weeks after surgery in the articular cartilage.
  • References (18)
  • Citations (4)
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References18
Newest
Published on Dec 1, 2015in Knee1.76
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences),
Hiromi Nakajima8
Estimated H-index: 8
(Ibaraki University)
+ 2 AuthorsMasataka Sakane26
Estimated H-index: 26
(University of Tsukuba)
Abstract Background The purpose of this study was to clarify the influence of mechanical unloading on histological changes of the patellar tendon (PT) insertion in rabbits. Materials and methods The PT was completely released from stress by drawing the patella toward the tibial tubercle with a stainless steel wire installed between the patella and tibial tubercle (mechanical unloading group, n = 28). The animals of the sham group underwent the same surgical procedure; however, the wire was not t...
Published on May 1, 2013in Journal of Anatomy2.64
Diogo Correa Maldonado2
Estimated H-index: 2
(UNIFESP: Federal University of São Paulo),
Marcelo Cavenaghi Pereira da Silva10
Estimated H-index: 10
(UNIFESP: Federal University of São Paulo)
+ 2 AuthorsRomeu Rodrigues de Souza13
Estimated H-index: 13
Studies have determined the effects of joint immobilization on the articular cartilage of sedentary animals, but we are not aware of any studies reporting the effects of joint immobilization in previously trained animals. The objective of the present study was to determine whether exercise could prevent degeneration of the articular cartilage that accompanies joint immobilization. We used light microscopy to study the thickness, cell density, nuclear size, and collagen density of articular carti...
Published on Jan 1, 2012in Knee Surgery, Sports Traumatology, Arthroscopy3.15
Masataka Sakane26
Estimated H-index: 26
(University of Tsukuba),
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences)
+ 4 AuthorsNaoyuki Ochiai31
Estimated H-index: 31
(University of Tsukuba)
Purpose The purpose of this study was to clarify the effects of partial resection on the glycosaminoglycan (GAG) layer thicknesses and chondrocyte turnover (apoptosis and cell proliferation) between uncalcified fibrocartilage (UF) and calcified fibrocartilage (CF) layers in an anterior cruciate ligament (ACL) insertion.
Published on Aug 1, 2010in Connective Tissue Research2.17
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences),
Masataka Sakane26
Estimated H-index: 26
(University of Tsukuba)
+ 3 AuthorsNaoyuki Ochiai31
Estimated H-index: 31
(University of Tsukuba)
The purpose of this study is to investigate cellular responses and histological changes of cartilaginous layers in human anterior cruciate ligament (ACL) tibial insertions after rupture compared with those in normal insertions. Fully 16 tibial insertions of ruptured ACLs were obtained during primary ACL reconstructions. We also obtained 16 normal ACL tibial insertions from cadavers. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) to detec...
Masataka Sakane26
Estimated H-index: 26
(University of Tsukuba),
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences)
+ 2 AuthorsNaoyuki Ochiai31
Estimated H-index: 31
(University of Tsukuba)
Background: The purpose of this study is to clarify the differences in time-dependent histological changes (chondrocyte apoptosis and glycosaminoglycan (GAG) layer thickness decrease) between uncalcified fibrocartilage (UF) and calcified fibrocartilage (CF) layers at the anterior cruciate ligament (ACL) insertion after ACL resection of rabbits. Methods: Forty male Japanese white rabbits underwent ACL substance resection in the right knee (resection group) and same operation without resection in ...
Published on Apr 30, 2007in Knee Surgery, Sports Traumatology, Arthroscopy3.15
Hirotaka Mutsuzaki11
Estimated H-index: 11
(University of Tsukuba),
Masataka Sakane26
Estimated H-index: 26
(University of Tsukuba)
+ 4 AuthorsNaoyuki Ochiai31
Estimated H-index: 31
(University of Tsukuba)
The purpose of this study is to investigate the histological changes and apoptosis of cartilaginous layers in human anterior cruciate ligament (ACL) tibial insertion at different time periods after rupture. By using a core reamer, 35 tibial insertions of ruptured ACLs were obtained during primary ACL reconstructions (number of days after injury: 19–206 days). A histological examination was performed and a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end la...
Published on Jan 1, 2007in Journal of Veterinary Medical Science0.91
Shinya Hattori1
Estimated H-index: 1
(TUAT: Tokyo University of Agriculture and Technology),
Masataka Sakane26
Estimated H-index: 26
(University of Tsukuba)
+ 3 AuthorsHiromi Nakajima1
Estimated H-index: 1
(TUAT: Tokyo University of Agriculture and Technology)
We examined time-dependent histological changes of the calcified fibrocartilage area in a tibial cranial cruciate ligament (CCL) insertion after ligament resection in rabbits. The animals were divided into two groups: those undergoing CCL substance resection in the right stifle (resected group) and those receiving the same operation without CCL resection in the left stifle (sham operated group). Five animals were euthanized with deep anaesthesia at four time periods (1, 2, 4 and 6 weeks), and Ha...
Published on Feb 1, 2006in Journal of Oral Pathology & Medicine2.03
Yuriko Hirota2
Estimated H-index: 2
,
Manabu Habu18
Estimated H-index: 18
+ 4 AuthorsJinichi Fukuda18
Estimated H-index: 18
Background: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthr...
Published on Nov 1, 2004in Clinical Orthopaedics and Related Research4.15
Diah S. Bramono6
Estimated H-index: 6
,
John C. Richmond40
Estimated H-index: 40
+ 2 AuthorsGregory H. Altman21
Estimated H-index: 21
Imbalance in the expression of matrix metalloproteinases and their inhibitors contribute considerably to abnormal connective tissue degradation prevalent in various orthopaedic joint diseases such as rheumatoid arthritis and osteoarthritis. Matrix metalloproteinase expression has been detected in li
Published on May 1, 2003in Archives of Physical Medicine and Rehabilitation2.70
Fujio Matsumoto4
Estimated H-index: 4
(U of O: University of Ottawa),
Guy Trudel22
Estimated H-index: 22
(U of O: University of Ottawa)
+ 1 AuthorsDavid Backman13
Estimated H-index: 13
(U of O: University of Ottawa)
Abstract Matsumoto F, Trudel G, Uhthoff HK, Backman DS. Mechanical effects of immobilization on the Achilles' tendon. Arch Phys Med Rehabil 2003;84:662-7. Objective: To measure the biomechanical effects of immobilization on the Achilles' tendon. Design: Experimental, controlled study. Setting: Physiatry research laboratory. Animals: Twenty adult rabbits. Intervention: One hindlimb immobilized in a cast for 4 weeks (n=10) or 8 weeks (n=10). The contralateral legs (n=20) served as controls. Main O...
Cited By4
Newest
Published on May 18, 2019in BMC Musculoskeletal Disorders2.00
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences),
Hiromi Nakajima8
Estimated H-index: 8
(Ibaraki University)
+ 1 AuthorsMasataka Sakane26
Estimated H-index: 26
Background A detailed evaluation focusing on the fibrocartilage layers in the anterior cruciate ligament (ACL) insertion is necessary to consider regeneration of the insertion. This study examined the development of the fibrocartilage layers in the ACL tibial insertion in rabbits by quantitative morphometric evaluations based on histological and immunohistochemical analyses.
Published on Jun 22, 2018in British Journal of Sports Medicine11.64
Alessio Bricca2
Estimated H-index: 2
,
Carsten Bogh Juhl15
Estimated H-index: 15
(UCPH: University of Copenhagen)
+ 2 AuthorsEwa M. Roos63
Estimated H-index: 63
Objective To investigate the impact of knee joint loading exercise on articular cartilage in people at risk of, or with established, knee osteoarthritis (OA) by conducting a systematic review of randomised controlled trials (RCTs). Design We followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses guidelines. Data sources We performed a literature search with no restriction on publication year or language in MEDLINE, EMBASE, CINAHL, the Cochrane Central Register of Control...
Published on May 1, 2019in Journal of Knee Surgery1.59
Richard Ma9
Estimated H-index: 9
(MU: University of Missouri),
Mark Stasiak5
Estimated H-index: 5
(HSS: Hospital for Special Surgery)
+ 1 AuthorsScott A. Rodeo60
Estimated H-index: 60
(HSS: Hospital for Special Surgery)
The purpose of this study is to establish a small animal anterior cruciate ligament (ACL) reconstruction research model where ACL graft force can be varied to create different graft force patterns with controlled knee motion. Cadaveric ( n = 10) and in vivo ( n = 10) rat knees underwent ACL resection followed by reconstruction using a soft tissue autograft. Five cadaveric and five in vivo knees received a nonisometric, high-force femoral graft tunnel position. Five cadaveric and five in vivo kne...
Published on Jan 1, 2018in Journal of Physical Therapy Science
Hirotaka Mutsuzaki11
Estimated H-index: 11
(Ibaraki Prefectural University of Health Sciences),
Hiromi Nakajima8
Estimated H-index: 8
(Ibaraki University),
Masataka Sakane26
Estimated H-index: 26
View next paperPreliminary experimental study on femural distal articular cartilage degeneration after anterior cruciate ligament transection in rabbits