Original paper
Sorting things out: Assessing effects of unequal specimen biomass on DNA metabarcoding
Abstract
Environmental bulk samples often contain many different taxa that vary several orders of magnitude in biomass. This can be problematic in DNA metabarcoding and metagenomic high‐throughput sequencing approaches, as large specimens contribute disproportionately high amounts of DNA template. Thus, a few specimens of high biomass will dominate the dataset, potentially leading to smaller specimens remaining undetected. Sorting of samples by specimen...
Paper Details
Title
Sorting things out: Assessing effects of unequal specimen biomass on DNA metabarcoding
Published Date
Jul 28, 2017
Journal
Volume
7
Issue
17
Pages
6918 - 6926
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Notes
History