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A non-destructive technique for chemical mapping of insect inclusions in amber

Published on Dec 1, 2018
· DOI :10.1007/s12542-018-0412-x
Anezka Popovski Kolaceke1
Estimated H-index: 1
(University of Regina),
Ryan C. McKellar12
Estimated H-index: 12
(KU: University of Kansas),
Mauricio Barbi1
Estimated H-index: 1
(University of Regina)
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Abstract
Synchrotron-based techniques offer a wealth of elemental, molecular, and structural insights in biological samples, but the application of these techniques to fossils is a relatively new development. Here we examine how synchrotron radiation micro X-ray fluorescence (SR µXRF) may be used to investigate the chemical composition of insects trapped in amber, while leaving the inclusions unaltered. Elemental distribution data could provide important information on tissue preservation in insect inclusions, as well as information on the processes involved in fossilization. By analyzing a series of ants (Hymenoptera: Formicidae) that range from modern material, to Eocene Baltic amber, and Late Cretaceous North Carolina amber, we investigate how variable preservation influences the results obtained through SR µXRF analyses, as well as the various merits and pitfalls associated with the application of this technique to amber inclusions. This work serves as an introduction to the underlying principles, strengths, and limitations associated with applying SR µXRF in a palaeontological context.
  • References (29)
  • Citations (1)
Cite
References29
Newest
Published on Dec 1, 2016in Current Biology9.19
Lida Xing18
Estimated H-index: 18
(China University of Geosciences),
Ryan C. McKellar5
Estimated H-index: 5
(University of Regina)
+ 11 AuthorsAlexander P. Wolfe47
Estimated H-index: 47
(U of A: University of Alberta)
Summary In the two decades since the discovery of feathered dinosaurs [1–3], the range of plumage known from non-avialan theropods has expanded significantly, confirming several features predicted by developmentally informed models of feather evolution [4–10]. However, three-dimensional feather morphology and evolutionary patterns remain difficult to interpret, due to compression in sedimentary rocks [9, 11]. Recent discoveries in Cretaceous amber from Canada, France, Japan, Lebanon, Myanmar, an...
Published on Jan 1, 2016in Current Biology9.19
Cédric Delevoye15
Estimated H-index: 15
(PSL Research University),
Xavier Heiligenstein4
Estimated H-index: 4
(PSL Research University)
+ 9 AuthorsVictor Faundez39
Estimated H-index: 39
(Emory University)
Recycling endosomes consist of a tubular network that emerges from vacuolar sorting endosomes and diverts cargoes toward the cell surface, the Golgi, or lysosome-related organelles. How recycling tubules are formed remains unknown. We show that recycling endosome biogenesis requires the protein complex BLOC-1. Mutations in BLOC-1 subunits underlie an inherited disorder characterized by albinism, the Hermansky-Pudlak Syndrome, and are associated with schizophrenia risk. We show here that BLOC-1 c...
Published on Mar 1, 2015in Journal of Synchrotron Radiation
Thomas Tolhurst1
Estimated H-index: 1
(University of Regina),
Mauricio Barbi1
Estimated H-index: 1
(University of Regina),
Tim Tokaryk1
Estimated H-index: 1
There is a great diversity of research being conducted at synchrotron facilities around the world and a diverse set of beamlines to accommodate this research. Time is a precious commodity at synchrotron facilities; therefore, methods that can maximize the time spent collecting data are of value. At the same time the incident radiation spectrum, necessary for some research, may not be known on a given beamline. A preliminary presentation of a method applicable to X-ray fluorescence spectrocopic a...
Published on Jan 1, 2015in Cretaceous Research2.12
Nathan Barling2
Estimated H-index: 2
(University of Portsmouth),
David M. Martill25
Estimated H-index: 25
(University of Portsmouth)
+ 1 AuthorsFlorence Gallien2
Estimated H-index: 2
(University of Portsmouth)
Fossil insects from the Lower Cretaceous (Aptian) Crato Formation of north-east Brazil are preserved as goethite replacements in laminated limestones of lacustro-lagoonal origin. They display remarkable degrees of morphological detail down to the macromolecular level in some examples. We document the fidelity of preservation and reveal an astonishing variety of morphological detail comparable in some instances with that found in amber inclusions.
Published on Nov 27, 2013
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University),
Wenxia Zheng10
Estimated H-index: 10
(NCSU: North Carolina State University)
+ 5 AuthorsSirine C. Fakra35
Estimated H-index: 35
(LBNL: Lawrence Berkeley National Laboratory)
The persistence of original soft tissues in Mesozoic fossil bone is not explained by current chemical degradation models. We identified iron particles (goethite-αFeO(OH)) associated with soft tissues recovered from two Mesozoic dinosaurs, using transmission electron microscopy, electron energy loss spectroscopy, micro-X-ray diffraction and Fe micro-X-ray absorption near-edge structure. Iron chelators increased fossil tissue immunoreactivity to multiple antibodies dramatically, suggesting a role ...
Dale E. Greenwalt5
Estimated H-index: 5
,
Yulia Goreva2
Estimated H-index: 2
(National Museum of Natural History)
+ 2 AuthorsRalph E. Harbach46
Estimated H-index: 46
(Natural History Museum)
Abstract Although hematophagy is found in ∼14,000 species of extant insects, the fossil record of blood-feeding insects is extremely poor and largely confined to specimens identified as hematophagic based on their taxonomic affinities with extant hematophagic insects; direct evidence of hematophagy is limited to four insect fossils in which trypanosomes and the malarial protozoan Plasmodium have been found. Here, we describe a blood-engorged mosquito from the Middle Eocene Kishenehn Formation in...
Published on Oct 1, 2013
Victor E. Krynicki1
Estimated H-index: 1
A small amber piece containing one nearly complete and four partial winged male fossil ants was collected from a lignite layer at a site along the Neuse River near Goldsboro, North Carolina, USA. Based on the anatomical details, these ants belong to the extinct subfamily Sphecomyrminae. While a formal species description and naming is not the purpose of this paper, similarities are noted to the ant genera Sphecomyrma and Baikurus, and taxonomic identification is made with the latter: Baikurus. T...
Published on Jan 1, 2013in Palaeontologia Electronica1.37
Hans Henderickx4
Estimated H-index: 4
(University of Antwerp),
Jan Bosselaers8
Estimated H-index: 8
(Royal Museum for Central Africa)
+ 2 AuthorsMatthieu Boone18
Estimated H-index: 18
(UGent: Ghent University)
Eocenoxenos palintropos gen. nov. et sp.nov., a new fossil strepsipteran taxon from Baltic amber is described. The position of the new genus is based on cladistic analyses of morphological data sets. Most data of the fossil where retrieved with 3D micro-CT scan reconstructions. The new taxon is unambiguously situated as a sister group of the Dundoxenos-Triozocera clade within the Corioxenidae. The eocene taxon combines derived characteristics typical of Corioxenidae with the posession of eight a...
Published on Dec 1, 2012in Zoological Journal of the Linnean Society2.91
Gregory D. Edgecombe43
Estimated H-index: 43
(Natural History Museum),
Varpu Vahtera8
Estimated H-index: 8
(Harvard University)
+ 4 AuthorsGonzalo Giribet60
Estimated H-index: 60
(Harvard University)
The first scolopocryptopid centipede known from the fossil record is a specimen of the subfamily Scolopocryptopinae in Miocene amber from Chiapas, southern Mexico. It is described here as Scolopocryptops simojovelensis sp. nov., displaying a distinct combination of morphological characters compared to extant congeners. Anatomical details of the fossil specimen were acquired by non-invasive 3D synchrotron microtomography using X-ray phase contrast. The phylogenetic position of the new species is ...
Published on Jul 19, 2012in Reviews in Analytical Chemistry2.88
Uwe Bergmann52
Estimated H-index: 52
,
P. M. Manning55
Estimated H-index: 55
,
Roy A. Wogelius22
Estimated H-index: 22
The application of the recently developed synchrotron rapid scanning X-ray fluorescence (SRS-XRF) technique to the mapping of large objects is the focus of this review. We discuss the advantages of SRS-XRF over traditional systems and the use of other synchrotron radiation (SR) techniques to provide corroborating spectroscopic and diffraction analyses during the same analytical session. After reviewing routine techniques used to analyze precious specimens, we present several case studies that sh...
Cited By1
Newest
Published on Nov 30, 2017in Cogent Chemistry
Tyler C. Borgwardt2
Estimated H-index: 2
(SDSM&T: South Dakota School of Mines and Technology),
Douglas P. Wells7
Estimated H-index: 7
(SDSM&T: South Dakota School of Mines and Technology)
AbstractThe idea of non-destructive elemental composition analysis is reviewed. The term non-destructive has many definitions in many different fields, as well as different definitions within a single field. The definition of non-destructive is discussed for several different fields: archeology, paleontology, forensics, space science, geochemistry. Activation analysis techniques, claimed to be used non-destructively in many fields, are used as a lens to provide a unique perspective on what non-d...