Enhanced Nucleation of Lysozyme Using Inorganic Silica Seed Particles of Different Sizes

In this work we investigate the impact of differently sized plain silica nanoparticles (NPs) between 10 and 200 nm on the crystallization of lysozyme (LSZ). In the first part of our work we investigate the electrostatic interactions between LSZ and NPs by zeta potential measurements and place special emphasis on the adsorption behavior of LSZ@SiO2. The determined adsorption isotherms—derived from UV–vis spectroscopy—indicate that with increasing...