Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus

Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae . Three single gene deletion mutants (Δ Mocapn7 , Δ Mocapn9 and Δ Mocapn14 ) and two double gene deletion mutants (Δ Mocapn4 Δ Mocapn7 and Δ Mocapn9 Δ Mocapn7 ) were obtained using the high-throughput gene...