Biology

Cytokine

Immunology

Signal transduction

CD40

In vitro

Flow cytometry

Biochemistry

Cytotoxic T cell

Cell biology

Receptor tyrosine kinase

MERTK

The Journal of Immunology

We have developed flow cytometry antibodies against three molecules expressed on tissue-resident mouse macrophages: MerTK, GPNMB (aka Osteoactivin, DC-HIL), and VSIG4 (aka CRIg). MerTK, a receptor tyrosine kinase, plays a crucial role in the clearance of apoptotic cells, as well as in the regulation of cytokine secretion and survival. MerTK is highly expressed by splenic red pulp macrophages, which aid in the clearance of apoptotic cells in the blood. GPNMB, a transmembrane glycoprotein, plays a role in negatively regulating cytokine secretion during inflammatory responses and is expressed on tissue-resident macrophages, including microglia. Finally, VSIG4, a B7 family-related complement receptor, negatively regulates T cell-mediated responses and is also expressed on tissue-resident macrophages. The three clones DS5MMER (MerTK), CTSREVL (GPNMB), and NLA14 (VSIG4), were validated using resident and thioglycollate-elicited peritoneal exudate cells. DS5MMER (MerTK) and NLA14 (VSIG4) were also validated by IHC u...

Regulation of MerTK, GPNMB, and VSIG4 on mouse macrophages