Capsaicin induced histological and ultrastructural changes in the submandibular salivary gland of albino rats

Published on Jun 1, 2016in Future Dental Journal
· DOI :10.1016/j.fdj.2016.04.002
Ahmed Mahmoud Halawa1
Estimated H-index: 1
(Ain Shams University),
Dahlia Ghazy Mohamed1
Estimated H-index: 1
(Ain Shams University),
Raneem Farouk Mohamed Adeeb Obeid1
Estimated H-index: 1
(Future University in Egypt)
Abstract Capsaicin is a pungent principle of hot red pepper. It is used in spices, food additives and drugs. In the present work, twenty rats were divided into two groups: control and capsaicin groups, each consisting of ten rats. The capsaicin group daily received a capsaicin dose equivalent to 0.1 mg/kg body weight dissolved in 0.5 ml distilled water by oro-oesophageal tube while the control group daily received 0.5 ml distilled water. After twenty one days, the submandibular salivary glands of both sides were excised, processed and examined histologically and ultrastructurally. Histological results revealed presence of pure mucous acini in the submandibular salivary gland. Some granular convoluted tubules showed degeneration while the excretory ducts showed loss of pseudostratification with the appearance of some flattened lining cells. Concerning the ultrastructural findings, some acinar cells showed dilated rough endoplasmic reticulum, other presented ultrastructural features similar to mucous acini. Granular convoluted tubules cells showed some irregular, shrunken nuclei with condensed chromatin. Their secretory granules were less electrondense than the control and presented ill-defined and fused outlines. Some of the excretory duct lining cells showed apically displaced irregular nuclei. One to two rows of flattened epithelial cells were observed apical to the lining cells. Vacuolizations, mitochondrial swelling and loss of cristae were detected in cells of some acini, granular convoluted tubules and excretory ducts. Most intercalated and striated duct cells showed ultrastructural features similar to that of control group. However, the basal part of some striated duct cells presented variable grades of mitochondrial affection. From the present work, it could be concluded that chronic capsaicin intake was associated with noticeable histological and ultrastructural changes in acini, granular convoluted tubules and excretory ducts of the SMSG in albino rats.
  • References (17)
  • Citations (2)
📖 Papers frequently viewed together
14 Citations
3 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1S ShoeybiH-Index: 1
#2M MasoumiH-Index: 1
Last. Asadollah AhmadikhahH-Index: 6
view all 6 authors...
3 Citations
#1A.O. Nwaopara (Ambrose Alli University)H-Index: 2
#2M.A. C. Odike (Ambrose Alli University)H-Index: 2
Last. G.I. Ewere (Ambrose Alli University)H-Index: 1
view all 5 authors...
Allergic reactions have been associated with cloves, black pepper and mast cells. The presence of mast cells and renal fibrosis has also been reported and in line with an ongoing investigation on the effects of excessive consumption of Yaji the complex Nigerian meat sauce, this study was designed to correlate the individual and combined effects of the excessive consumption of Yaji-spices (ginger, clove, red pepper and black pepper) on the histology of the kidney, using 12 adult rabbits. The anim...
15 CitationsSource
#1Masahiko Mori (Asahi University)H-Index: 26
#2Shinichiro Sumitomo (Asahi University)H-Index: 11
Last. Michio Shikimori (Asahi University)H-Index: 5
view all 6 authors...
Salivary glands secrete saliva which contains mucins, antimicrobial substances and growth factors. Since epidermal growth factor (EGF) and nerve growth factor (NGF) were demonstrated in murine submandibular glands (SMGs), several growth factors and biologically-active peptides have been studied in the human or other mammalian salivary glands and saliva. These growth factors may have a functional role in cell migration, proliferation and maturation within not only salivary glands but also other o...
10 CitationsSource
#1Filomena Conforti (University of Calabria)H-Index: 29
#2Giancarlo A. Statti (University of Calabria)H-Index: 32
Last. Francesco Menichini (University of Calabria)H-Index: 36
view all 3 authors...
Abstract The aim of the present work was to evaluate the chemical composition and the radical-scavenging and antioxidant activities of hot pepper fruits ( Capsicum annuum L. var. acuminatum ) at three maturity stages (small green, green and red). GC–MS analysis of n -hexane and chloroform fractions showed a different composition between the three stages of ripening. The first stage of maturation (small green) showed the highest radical-scavenging activity (IC 50 of 129 μg/ml). Using the bovine b...
125 CitationsSource
#1George F. Antonious (Kentucky State University)H-Index: 22
#2Tejinder S. Kochhar (Kentucky State University)H-Index: 5
Last. John C. Snyder (UK: University of Kentucky)H-Index: 21
view all 4 authors...
The U.S. Department of Agriculture (USDA) pepper (Capsicum spp.) germplasm collection contains several thousand members or accessions. Many of these species and cultivars have not been analyzed for their concentrations of ascorbic acid, capsaicin, and total phenolic compounds, which are important antioxidants having a number of benefits for human health. The objective of this investigation was to select candidate accessions of hot pepper having high concentrations of ascorbic acid, capsaicin, fr...
43 CitationsSource
#1M. N. Satyanarayana (CSIR-CFTRI: Central Food Technological Research Institute)H-Index: 1
In recent years, infection of the stomach with the organism Helicobacter Pylori has been found to be the main cause of gastric ulcers, one of the common ailments afflicting humans. Excessive acid secretion in the stomach, reduction in gastric mucosal blood flow, constant intake of non-steroid anti-inflammatory drugs (NSAIDS), ethanol, smoking, stress etc. are also considered responsible for ulcer formation. The prevalent notion among sections of population in this country and perhaps in others i...
58 CitationsSource
#1Gyula MózsikH-Index: 22
#2Szolcsanyi JanosH-Index: 39
Last. István RáczH-Index: 14
view all 3 authors...
AIM: To evaluate the gastro-protective effect of capsaicin against the ethanol- and indomethacin (IND)-induced gastric mucosal damage in healthy human subjects. METHODS: The effects of small doses (1-8 μg/mL, 100 mL) of capsaicin on the gastric acid secretion basal acid output (BAO) and its electrolyte concentration, gastric transmucosal potential difference (GTPD), ethanol- (5 mL 300 mL/L i.g.) and IND- (3×25 mg/d) induced gastric mucosal damage were tested in a randomized, prospective study of...
55 CitationsSource
#1Hiroyuki Nishikawa (Kindai University)H-Index: 13
#2Atsufumi Kawabata (Kindai University)H-Index: 40
Proteinase-activated receptor (PAR)1 and PAR2 are abundantly expressed in the gastric mucosa and smooth muscle. PAR2 modulates a variety of gastric functions, mediating secretion of mucus and pepsinogen, suppression of acid output, an increase in mucosal blood flow, and smooth muscle contraction/relaxation. PAR2 is considered primarily protective in the gastric mucosa. PARs other than PAR2 might also be involved in the modulation of gastric functions. PARs, especially PAR2, would thus appear to ...
6 CitationsSource
#1Hideo Katsukawa (Asahi University)H-Index: 10
#2Y. Shang (Kyushu University)H-Index: 1
Last. Tadataka Sugimura (Asahi University)H-Index: 4
view all 10 authors...
Abstract Dietary capsaicin consumed by rats over several days induces cystatin-like substances in submandibular saliva. Yet the physiological role of these salivary proteins has not been thoroughly investigated. Salivary cystatins in the rat submandibular glands are known to be induced by chronic treatment with the sympathetic beta-agonist, isoproterenol. In the present study, the possible roles of the salivary proteins on food intake were examined by comparing consumption of a capsaicin-adulter...
8 CitationsSource
#1Hideo Katsukawa (Asahi University)H-Index: 10
#2Yuzo Ninomiya (Asahi University)H-Index: 48
Irritating dietary substances such as tannin and papain have been reported to alter the morphology of salivary glands and their secretions. Such alterations can be one line of protection from toxic or irritating substances in food. We investigated the effects of dietary capsaicin (a pungent ingredient of hot red pepper) on the rat submandibular gland and its secretions. Several groups of animals were offered either control diets or diets containing capsaicin (from 0.0001 to 0.1%) for seven days....
21 CitationsSource
Cited By2
#2Dina Sabry (Cairo University)H-Index: 20
Last. Safa Fathy (Cairo University)
view all 4 authors...
ABSTRACTSalivary gland neoplasms exhibit complex histopathology in a variety of tumor types and treatment options depend largely on the stage of the cancer. Induced pluripotent stem cells (iPS) have been investigated for treating induced salivary gland cancer and for restoring salivary gland function. We investigated iPS treatment for salivary gland cancer both in vitro and in vivo. For our study in vitro, we re-programmed human skin fibroblasts to form iPS cells using a plasmid containing Oct4,...