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Withania somnifera Root Extract Enhances Telomerase Activity in the Human HeLa Cell Line

Published on Jan 1, 2016in Advances in Bioscience and Biotechnology
· DOI :10.4236/abb.2016.74018
Vasantharaja Raguraman1
Estimated H-index: 1
,
Jamuna R. Subramaniam11
Estimated H-index: 11
Sources
Abstract
Aging is a decelerating unidirectional process of life. Shortening of telomeric DNA, the (TTAGGG)n hexanucleotide repeats, which form the caps at the chromosome ends, is implicated to determine the aging process, and more importantly the healthy lifespan itself. Telomerase, a ribonucleoprotein having reverse transcriptase activity, arrests telomere loss through addition of the TTAGGG repeats de novo, to the ends of the chromosome. The telomere/telomerase maintenance is an inevitable necessity to delay aging and for a healthy lifespan. Here, we report the potential of full-spectrum, high concentration Ashwagandha (Withania somnifera), an Ayurvedic medicinal herb, root extract to increase telomerase activity. HeLa cells, when treated with various concentrations of Ashwagandha root extract, showed an increase in telomerase activity measured with the established Telomerase Rapid Amplification Protocol (TRAP) assay. Ashwagandha root extract increased telomerase activity with highest enhancement of ~45% at 10 - 50 μg concentration. Thus, Ashwagandha root extract has the anti-aging inducing potential.
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References23
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#1Kunlin JinH-Index: 64
#2James W. Simpkins (WVU: West Virginia University)H-Index: 49
Last. Ilia Stambler (BIU: Bar-Ilan University)H-Index: 7
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Due to the aging of the global population and the derivative increase in aging-related non-communicable diseases and their economic burden, there is an urgent need to promote research on aging and aging-related diseases as a way to improve healthy and productive longevity for the elderly population. To accomplish this goal, we advocate the following policies: 1) Increasing funding for research and development specifically directed to ameliorate degenerative aging processes and to extend healthy ...
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#1P. C. Prabu (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 1
#2S. Panchapakesan (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 1
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Withania somnifera is a widely used medicinal plant for several disorders. Toxicity studies on Withania somnifera are not available. Acute and sub-acute oral toxicities of Withania somnifera root extract in Wistar rats were evaluated in the present study. In the acute toxicity study, WSR extract was administered to five rats at 2000 mg/kg, once orally and were observed for 14 days. No toxic signs/mortality were observed. In the sub-acute study, WSR extract was administered once daily for 28 days...
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#1Ranjeet KumarH-Index: 2
#2Kuldeep GuptaH-Index: 2
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Background: In the ancient Indian herbal medicine system several ayurvedic preparations are claimed to have longevity enhancing effects. But, so far, no clear scientific evidence has been provided. One among them, is the roots of the plant, commonly known as Ashwagandha (Withania somnifera Dunal- WSD), which is supposed to have myriad of beneficial effects including long life. Purpose: Here, we evaluated both the root extract (RE) and its purified ingredients (PI-RE) with a similar composition a...
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Although inhibition of the TOR signalling pathway extends lifespan in invertebrates, it was unknown whether mTOR signalling inhibition has similar effects in mammalian species. Here, feeding mice the drug rapamycin — an inhibitor of the mTOR pathway — late in life is shown to extend lifespan by 9–14%; currently, the only way to extend lifespan in rodents is by severe dietary restriction.
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Abstract Deregulated apoptosis and suppressed tumour reactive immunity render tumour cells to grow amok in the host body. Traditionally used botanicals may offer potential anticancer chemo-immunotherapeutic leads. We report in this study a chemically standardised herbal formulation (WSF) of Withania somnifera possessing anticancer and Th1 immune up-regulatory activities. WSF produced cytotoxicity in a panel of human cancer cell lines in vitro . The molecular mechanism of cell cytotoxicity, IC 50...
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