Withania somnifera Root Extract Enhances Telomerase Activity in the Human HeLa Cell Line

Published on Jan 1, 2016in Advances in Bioscience and Biotechnology
· DOI :10.4236/abb.2016.74018
Vasantharaja Raguraman1
Estimated H-index: 1
Jamuna R. Subramaniam11
Estimated H-index: 11
Aging is a decelerating unidirectional process of life. Shortening of telomeric DNA, the (TTAGGG)n hexanucleotide repeats, which form the caps at the chromosome ends, is implicated to determine the aging process, and more importantly the healthy lifespan itself. Telomerase, a ribonucleoprotein having reverse transcriptase activity, arrests telomere loss through addition of the TTAGGG repeats de novo, to the ends of the chromosome. The telomere/telomerase maintenance is an inevitable necessity to delay aging and for a healthy lifespan. Here, we report the potential of full-spectrum, high concentration Ashwagandha (Withania somnifera), an Ayurvedic medicinal herb, root extract to increase telomerase activity. HeLa cells, when treated with various concentrations of Ashwagandha root extract, showed an increase in telomerase activity measured with the established Telomerase Rapid Amplification Protocol (TRAP) assay. Ashwagandha root extract increased telomerase activity with highest enhancement of ~45% at 10 - 50 μg concentration. Thus, Ashwagandha root extract has the anti-aging inducing potential.
Figures & Tables
  • References (23)
  • Citations (4)
📖 Papers frequently viewed together
5 Authors (Ankur Patel, ..., Angelo Messina)
1 Citations
2 Citations
9 Citations
78% of Scinapse members use related papers. After signing in, all features are FREE.
#1Kunlin JinH-Index: 64
#2James W. Simpkins (WVU: West Virginia University)H-Index: 49
Last. Ilia Stambler (BIU: Bar-Ilan University)H-Index: 7
view all 5 authors...
Due to the aging of the global population and the derivative increase in aging-related non-communicable diseases and their economic burden, there is an urgent need to promote research on aging and aging-related diseases as a way to improve healthy and productive longevity for the elderly population. To accomplish this goal, we advocate the following policies: 1) Increasing funding for research and development specifically directed to ameliorate degenerative aging processes and to extend healthy ...
63 CitationsSource
#1P. C. Prabu (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 1
#2S. Panchapakesan (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 1
Last. C David Raj (SASTRA: Shanmugha Arts, Science, Technology & Research Academy)H-Index: 4
view all 3 authors...
Withania somnifera is a widely used medicinal plant for several disorders. Toxicity studies on Withania somnifera are not available. Acute and sub-acute oral toxicities of Withania somnifera root extract in Wistar rats were evaluated in the present study. In the acute toxicity study, WSR extract was administered to five rats at 2000 mg/kg, once orally and were observed for 14 days. No toxic signs/mortality were observed. In the sub-acute study, WSR extract was administered once daily for 28 days...
28 CitationsSource
#1Ranjeet KumarH-Index: 2
#2Kuldeep GuptaH-Index: 2
Last. Jamuna R. SubramaniamH-Index: 11
view all 5 authors...
Background: In the ancient Indian herbal medicine system several ayurvedic preparations are claimed to have longevity enhancing effects. But, so far, no clear scientific evidence has been provided. One among them, is the roots of the plant, commonly known as Ashwagandha (Withania somnifera Dunal- WSD), which is supposed to have myriad of beneficial effects including long life. Purpose: Here, we evaluated both the root extract (RE) and its purified ingredients (PI-RE) with a similar composition a...
10 CitationsSource
#1Kopal Saharia (IITK: Indian Institute of Technology Kanpur)H-Index: 2
#2Upasna Arya (IITK: Indian Institute of Technology Kanpur)H-Index: 4
Last. Jamuna R. Subramaniam (IITK: Indian Institute of Technology Kanpur)H-Index: 11
view all 8 authors...
Abstract Aging is a debilitating process often associated with chronic diseases such as diabetes, cardiovascular and neurodegenerative diseases like Alzheimer's disease (AD). AD occurs at a very high incidence posing a huge burden to the society. Model organisms such as C. elegans become essential to understand aging or lifespan extension – the etiology, molecular mechanism and identification of new drugs against age associated diseases. The AD model, manifesting Aβ proteotoxicity, in C. elegans...
25 CitationsSource
Last. Maria A. BlascoH-Index: 84
view all 6 authors...
Summary Here, we show that a small-molecule activator of telomerase (TA-65) purified from the root of Astragalus membranaceus is capable of increasing average telomere length and decreasing the percentage of critically short telomeres and of DNA damage in haploinsufficient mouse embryonic fibroblasts (MEFs) that harbor critically short telomeres and a single copy of the telomerase RNA Terc gene (G3 Terc + ⁄ ) MEFs). Importantly, TA-65 does not cause telomere elongation or rescue DNA damage in si...
134 CitationsSource
#1Mariela Jaskelioff (Harvard University)H-Index: 6
#2Florian L. MullerH-Index: 34
Last. Ronald A. DePinhoH-Index: 147
view all 13 authors...
An ageing world population has fuelled interest in regenerative remedies that may stem declining organ function and maintain fitness. Unanswered is whether elimination of intrinsic instigators driving age-associated degeneration can reverse, as opposed to simply arrest, various afflictions of the aged. Such instigators include progressively damaged genomes. Telomerase-deficient mice have served as a model system to study the adverse cellular and organismal consequences of wide-spread endogenous ...
460 CitationsSource
#1David E. HarrisonH-Index: 4
#2Randy Strong (University of Texas Health Science Center at San Antonio)H-Index: 42
Last. Richard A. Miller (UM: University of Michigan)H-Index: 59
view all 14 authors...
Although inhibition of the TOR signalling pathway extends lifespan in invertebrates, it was unknown whether mTOR signalling inhibition has similar effects in mammalian species. Here, feeding mice the drug rapamycin — an inhibitor of the mTOR pathway — late in life is shown to extend lifespan by 9–14%; currently, the only way to extend lifespan in rodents is by severe dietary restriction.
2,139 CitationsSource
#1Fayaz Malik (CSIR: Council of Scientific and Industrial Research)H-Index: 21
#2Ajay Kumar (CSIR: Council of Scientific and Industrial Research)H-Index: 17
Last. Jaswant Singh (CSIR: Council of Scientific and Industrial Research)H-Index: 25
view all 13 authors...
Abstract Deregulated apoptosis and suppressed tumour reactive immunity render tumour cells to grow amok in the host body. Traditionally used botanicals may offer potential anticancer chemo-immunotherapeutic leads. We report in this study a chemically standardised herbal formulation (WSF) of Withania somnifera possessing anticancer and Th1 immune up-regulatory activities. WSF produced cytotoxicity in a panel of human cancer cell lines in vitro . The molecular mechanism of cell cytotoxicity, IC 50...
43 CitationsSource
#2Ignacio FloresH-Index: 24
Last. Maria A. BlascoH-Index: 84
view all 13 authors...
Summary Telomerase confers limitless proliferative potential to most human cells through its ability to elongate telomeres, the natural ends of chromosomes, which otherwise would undergo progressive attrition and eventually compromise cell viability. However, the role of telomerase in organismal aging has remained unaddressed, in part because of the cancer-promoting activity of telomerase. To circumvent this problem, we have constitutively expressed telomerase reverse transcriptase (TERT), one o...
308 CitationsSource
#1Deepti Srivastava (IITs: Indian Institutes of Technology)H-Index: 1
#2Upasna Arya (IITs: Indian Institutes of Technology)H-Index: 4
Last. Jamuna R. Subramaniam (IITs: Indian Institutes of Technology)H-Index: 11
view all 6 authors...
Though the lifespan extension mechanism is partly understood from C. elegans to mice, a viable pharmacological intervention is not yet feasible. Here, we report that reserpine largely known as an antipsychotic–antihypertensive drug, can extend C. elegans lifespan. Chronic reserpine treatment from embryo stage or young adults extends the C. elegans lifespan robustly at 25°C. Most importantly, the reserpine treated long lived worms are active (locomotion and pharyngeal pumping) for a long time the...
36 CitationsSource
Cited By4
#1Deepa S. Mandlik (Poona College of Arts Science & Commerce)
#2Ajay Gajanan Namdeo (Poona College of Arts Science & Commerce)H-Index: 4
Withania somnifera, commonly known as “Ashwagandha” or “Indian ginseng” is an essential therapeutic plant of Indian subcontinent regions. It is regularly used, alone or in combination with other pl...
#1Bianca Maria Baroli (University of Cagliari)H-Index: 10
#2Eleonora Loi (University of Cagliari)H-Index: 2
Last. Patrizia Zavattari (University of Cagliari)H-Index: 19
view all 10 authors...
Oxidative stress is commonly observed in both idiopathic and genetic cases of Parkinson’s disease (PD). It plays an important role in the degeneration of dopaminergic neurons, and it has been associated with altered telomere length (TL). There is currently no cure for PD, and extracts of antioxidative plant, such as Mucuna pruriens and Withania somnifera, are commonly used in Ayurveda to treat patients with PD. In this study, we evaluated 2 enzymatic markers of oxidative stress, glutathione (GSH...
#1Hafize Uzun (Istanbul University)H-Index: 35
Inflammation plays a role in the etiology of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s diseases, which have the highest mortality rates. It has been argued that the lifetime of macromolecular damage caused by chronic inflammation can be the cause of aging process. There are studies indicating that aging is also closely related with low-grade chronic inflammation, defined as sterile inflammation. Another definition of sterile inflammation is pathogen-free infla...
#1Komal Saraswat (Allahabad University)H-Index: 1
#2Syed Ibrahim Rizvi (Allahabad University)H-Index: 27
ABSTRACTIntroduction: Scientific achievements in the last few decades, leading to effective therapeutic interventions, have dramatically improved human life expectancy. Consequently, aging has become a significant problem and represents the major risk factor for most human pathologies including diabetes, cardiovascular diseases, neurological disorders, and cancer. Scientific discoveries over the past two decades have been instrumental in dissecting molecular mechanism(s) which play important rol...
19 CitationsSource
#1Niraj TripathiH-Index: 1
#2Divya Shrivastava (Jaipur National University)H-Index: 3
Last. Prakash S. Bisen (Jaipur National University)H-Index: 3
view all 7 authors...
Abstract Background Withania somnifera, a high value medicinal plant is a major source of pharmaceutically important active compounds withanolides. Withania somnifera has been used in ayurveda as health restorative and anabolic agent besides having anti-arthritic, antidepressant, anti-microbial, anti-inflammatory, anti-diabetic, anti-stress, neuroprotective and cardio-protective activities. Hypothesis/Purpose The mining of the compound(s) of interest offers opportunity to identify desired attrib...
3 CitationsSource