Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples
Abstract
Subcellular lipidomics is a novel field of research that requires the careful combination of several pre-analytical and analytical steps. To define a reliable strategy for mitochondrial lipid profiling, we performed a systematic comparison of different mitochondria isolation procedures by western blot analyses and comprehensive high-resolution lipidomics. Using liver-derived HepG2 cells, we compared three common mitochondria isolation methods,...
Paper Details
Title
Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples
Published Date
Feb 19, 2016
Journal
Volume
6
Issue
1
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