Telomere measurement by quantitative PCR
Abstract
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the...
Paper Details
Title
Telomere measurement by quantitative PCR
Published Date
May 15, 2002
Journal
Volume
30
Issue
10
Pages
47e - 47
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