Original paper
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Abstract
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the microinjection of endonucleases into embryos requires a high skill level, is time consuming, and may cause damage to embryos. Here, we demonstrate...
Paper Details
Title
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
Published Date
Oct 1, 2014
Journal
Volume
4
Issue
1
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Notes
History