Mechanisms of epithelial translocation of the 2-gliadin-33mer in coeliac sprue

Background and aims:

The α₂-gliadin-33mer has been shown to be important in the pathogenesis of coeliac disease. We aimed to study mechanisms of its epithelial translocation and processing in respect to transcytotic and paracellular pathways.

Methods:

Transepithelial passage of a fluorescence-labelled α₂-gliadin-33mer was studied in Caco-2 cells by using reverse-phase high-performance liquid...