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Tetramethylpyrazine inhibits activities of glioma cells and glutamate neuro-excitotoxicity: Potential therapeutic application for treatment of gliomas

Published on Apr 1, 2008in Neuro-oncology10.09
· DOI :10.1215/15228517-2007-051
Yu-Show Fu13
Estimated H-index: 13
(NYMU: National Yang-Ming University),
Yen Yang Lin1
Estimated H-index: 1
(NYMU: National Yang-Ming University)
+ 8 AuthorsJia Yi Wang1
Estimated H-index: 1
(NDU: National Defense University)
Cite
Abstract
We tested the herbal extract 2,3,5,6-tetramethylpyrazine (TMP) for possible therapeutic efficacy against a glioma cell line and against gliomas transplanted into rat brains. In the cultured glioma cells, 50 μM TMP significantly inhibited glutamate-induced increase in intracellular calcium. Significant cell damage (30%) and proliferation suppression (10%), however, occurred only at higher concentrations (200–400 μM). Glioma- neuronal co-culturing resulted in significant neuronal damage and higher proliferation of the glioma cells (140%) compared with single cultures. Low concentrations of TMP (⩽200 μM) attenuated the neuronal damage, suppressed glioma migration, and decreased glioma proliferation in the neuronal-glioma co-culture. Gliomas transplanted into the frontal cortical area exhibited high proliferation, with untreated rats dying 10–23 days later. TMP treatment inhibited tumor growth and significantly extended survival time. The results indicate that TMP can suppress glioma activity, including growth, and protect neurons against glioma-induced excitotoxicity, suggesting that TMP may have therapeutic potential in the treatment of malignant gliomas.
  • References (43)
  • Citations (30)
Cite
References43
Newest
Published on Feb 1, 2006in Neurochemistry International3.99
Tsung-Kuei Kao9
Estimated H-index: 9
(NU: Nanjing University),
Yen-Chuan Ou15
Estimated H-index: 15
+ 7 AuthorsWen-Huang Peng24
Estimated H-index: 24
(PRC: China Medical University (PRC))
Abstract In traditional Chinese medicine, Ligusticum wallichii Franchat (Chuan Xiong) and its active ingredient tetramethylpyrazine (TMP) have been used to treat cardiovascular diseases and to relieve various neurological symptoms such as ischemic deficits. However, scientific evidence related to their effectiveness or precise modes of neuroprotective action is largely unclear. In the current study, we elicited the neuroprotective mechanisms of TMP after focal cerebral ischemic/reperfusion (I/R)...
Published on Feb 1, 2006in Planta Medica2.75
Georgehsiao31
Estimated H-index: 31
,
Yi Cheng Chen3
Estimated H-index: 3
(TMU: Taipei Medical University)
+ 4 AuthorsJoen Rong Sheu36
Estimated H-index: 36
Tetramethylpyrazine (TMPZ) is an active ingredient isolated from a commonly used Chinese herb, Ligusticum wallichii Franchat, which has long been used in China for the treatment of vascular diseases. In the present study, TMPZ significantly attenuated middle cerebral artery occlusion (MCAO)-induced focal cerebral ischemia in rats. Administration of TMPZ at 10 and 20 mg/ kg produced concentration-dependent reductions in infarct size compared to that of control rats. MCAO-induced focal cerebral is...
Published on Jul 1, 2005in Neuro-oncology10.09
Antonella Arcella28
Estimated H-index: 28
,
G. Carpinelli17
Estimated H-index: 17
(ISS: Istituto Superiore di Sanità)
+ 7 AuthorsFerdinando Nicoletti75
Estimated H-index: 75
U87MG human glioma cells in cultures expressed metabotropic glutamate (mGlu) receptors mGlu2 and mGlu3. Addition of the mGlu2/3 receptor antagonist LY341495 to the cultures reduced cell growth, expression of cyclin D1/2, and activation of the MAP kinase and phosphatidylinositol-3-kinase pathways. This is in line with the evidence that activation of mGlu2/3 receptors sustains glioma cell proliferation. U87MG cells were either implanted under the skin (1 × 106 cells/0.5 ml) or infused into the cau...
Kar-Lok Wong17
Estimated H-index: 17
(TMU: Taipei Medical University),
Paul Chan23
Estimated H-index: 23
(TMU: Taipei Medical University)
+ 5 AuthorsJuei-Tang Cheng7
Estimated H-index: 7
(NCKU: National Cheng Kung University)
1. Tetramethylpyrazine (TMP) is one of the active principles contained in Ligusticum chuanxiong Hort. (Umbelliferae), a herb that has been used widely in China to treat vascular disorders. 2. In an attempt to elucidate the possible mechanisms of action of TMP, the effect of TMP on intracellular calcium concentrations ([Ca 2 + ] i ) was investigated in cultured vascular smooth muscle (A7r5) cells using the Ca 2 + -sensitive dye Fura-2 as an indicator. 3. The increase in [Ca 2 + ] i in A7r5 cells ...
Published on May 1, 2003in European Journal of Pharmacology3.17
Alice Lai Shan Au10
Estimated H-index: 10
(CUHK: The Chinese University of Hong Kong),
Yiu Wa Kwan25
Estimated H-index: 25
(CUHK: The Chinese University of Hong Kong)
+ 2 AuthorsGuo-Wei He29
Estimated H-index: 29
(CUHK: The Chinese University of Hong Kong)
Abstract In this study, we have evaluated the underlying mechanisms responsible for the relaxation response of ligustrazine (2,3,5,6-tetra-methyl-pyrazine; 2,3,5,6-MP) and its structural analogues (2-methyl-pyrazine (2-MP); ethyl-pyrazine (EP); 2,3-di-methyl-pyrazine (2,3-MP); 2,5-di-methyl-pyrazine (2,5-MP); 2,6-di-methyl-pyrazine (2,6-MP) and 2,3,5-tri-methyl-pyrazine (2,3,5-MP)) in porcine left anterior descending coronary artery (tertiary branch, O.D. ≤1 mm). In 5-hydroxytryptamine (3 μM) pr...
Published on Apr 1, 2003in Life Sciences3.45
Zhaohui Zhang3
Estimated H-index: 3
(WHU: Wuhan University),
Taotao Wei18
Estimated H-index: 18
(CAS: Chinese Academy of Sciences)
+ 3 AuthorsWenjuan Xin21
Estimated H-index: 21
(CAS: Chinese Academy of Sciences)
Abstract Tetramethylpyrazine is one of the active ingredients of the Chinese herb Ligusticum wallichii Franchat. By electron spin resonance spin trapping methods, effects of tetramethylpyrazine on superoxide anion and nitric oxide generated by human polymorphonuclear leukocytes were studied. During the respiratory burst of polymorphonuclear leukocytes induced by N-formylmethionyl-leucyl-phenylalanine, tetramethylpyrazine scavenges superoxide anion dose-dependently, and decreases the production o...
Published on Apr 1, 2003in European Journal of Pharmacology3.17
Zhaohui Zhang3
Estimated H-index: 3
(WHU: Wuhan University),
Taotao Wei18
Estimated H-index: 18
(CAS: Chinese Academy of Sciences)
+ 3 AuthorsWenjuan Xin21
Estimated H-index: 21
(CAS: Chinese Academy of Sciences)
Tetramethylpyrazine and ferulic acid are two active ingredients of a Chinese herbal medicine Ligusticum wallichi Franchat. In the present investigation, iron-induced oxidative neuronal damage and the protective effects of tetramethylpyrazine and ferulic acid against this induction were studied in primary cultures of rat cerebellar granule cells. When neurons were treated with 200 AM of FeSO4 for 1 h, lipid peroxidation in neurons increased time dependently, as measured with the thiobarbituric ac...
Published on Sep 1, 2002in Nature Medicine30.64
Shogo Ishiuchi14
Estimated H-index: 14
(Gunma University),
Keisuke Tsuzuki6
Estimated H-index: 6
(Gunma University)
+ 9 AuthorsMasaru Tamura18
Estimated H-index: 18
(Gunma University)
Blockage of Ca 2+ -permeable AMPA receptors suppresses migration and induces apoptosis in human glioblastoma cells
Published on Mar 1, 2002in Neuroreport1.15
Yang-Hsin Shih25
Estimated H-index: 25
,
Se-Ling Wu1
Estimated H-index: 1
+ 3 AuthorsYu-Show Fu13
Estimated H-index: 13
Tetramethylpyrazine (TMP) is the major component extracted from the Chinese herb, Chuanxiong. This study focuses on the protective effect of tetramethylpyrazine in kainate-induced excitotoxicity in rat hippocampus. Primary neuronal cultures raised from cells isolated from the hippocampi of 7-day old
Published on Mar 1, 2002in Microvascular Research2.60
Hong Zhao1
Estimated H-index: 1
(CNRS: Centre national de la recherche scientifique),
Xi Dong2
Estimated H-index: 2
(China-Japan Friendship Hospital)
+ 4 AuthorsJinning Lou23
Estimated H-index: 23
(University of Geneva)
An in vitro model with micropipette technique was used to investigate single-cell adhesion probability between lymphocytes and endothelial cells. The basal adhesion probability between lymphocytes and endothelial cells was low and was significantly increased when either lymphocytes were activated by phytohemagglutinin (PHA) or endothelial cells were stimulated by tumor necrosis factor. The adhesion probability of lymphocytes to human umbilical vein endothelial cells was similar to that of lympho...
Cited By30
Newest
Published on Sep 1, 2018in Journal of Functional Foods3.20
Jui Cheng Tsai2
Estimated H-index: 2
(NTNU: National Taiwan Normal University),
Wen Shan Liu1
Estimated H-index: 1
(NTNU: National Taiwan Normal University)
+ 5 AuthorsYun-Ju Lai8
Estimated H-index: 8
(NTNU: National Taiwan Normal University)
Abstract Glioblastoma multiforme (GBM), a highly recurrent malignant disease, is a grade IV glioma with poor prognosis. Glioblastoma stem cells (GSCs) are the new potential target for GBM intervention. To develop an improved treatment by targeting GSCs, we identified neriifolin, an active principle in extracts of Cerbera manghas L., as an effective inhibitor for the growth and migration of GBM cell lines and their tumorspheres. In this study, neriifolin effectively inhibited the growth and colon...
Published on Jan 1, 2018in Biological & Pharmaceutical Bulletin1.54
Chuiliang Liu6
Estimated H-index: 6
,
Zhipeng Li + 4 AuthorsYujuan Li5
Estimated H-index: 5
(SYSU: Sun Yat-sen University)
Published on Feb 1, 2017in Oncology Reports3.04
Yi Zhou3
Estimated H-index: 3
(Peking Union Medical College Hospital),
Zhigang Ji6
Estimated H-index: 6
(Peking Union Medical College Hospital)
+ 3 AuthorsYu Xiao3
Estimated H-index: 3
(Peking Union Medical College Hospital)
Published on Sep 8, 2016in Investigative Ophthalmology & Visual Science3.81
Xiaoxiao Cai5
Estimated H-index: 5
(SYSU: Sun Yat-sen University),
YangfanYang6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
+ 4 AuthorsMinbin Yu15
Estimated H-index: 15
(SYSU: Sun Yat-sen University)
Published on Jul 8, 2016in PLOS ONE2.78
Shanshan Wang1
Estimated H-index: 1
,
Ting Lei1
Estimated H-index: 1
,
Man Zhang1
Estimated H-index: 1
Chemotherapy is an important strategy for the treatment of bladder cancer. However, the main problem limiting the success of chemotherapy is the development of multidrug resistance (MDR). To improve the management of bladder cancer, it is an urgent matter to search for strategies to reverse MDR. We chose three kinds of herbal medicines including ginsenoside Rh2, (-)-Epigallocatechin gallate (EGCG) and Tetramethylpyrazine (TMP) to detect their effects on bladder cancer. Reversal effects of these ...
Published on Jan 1, 2016in Iranian Journal of Pharmaceutical Research1.18
Wei Chen3
Estimated H-index: 3
,
Weixiong Chen2
Estimated H-index: 2
+ 2 AuthorsYunmin Lu3
Estimated H-index: 3
The purpose of the current study was to evaluate the anti-inflammatory activity of tetramethlpyrazine on oxazolone-induced colitis mice. Spleen mononuclear cells (SMC), lamina propria mononuclear cells (LPMC) and peripheral blood mononuclear cells (PBMC) were isolated from oxazolone-induced colitis and normal mice. The colitis cells treated by oxazolone were randomly divided into model, low dose, middle dose and high dose groups treated with 0, 0.5, 1.0 and 2.0 g/L tetramethlpyrazine, respective...
Published on Dec 1, 2015in Molecular Medicine Reports1.85
Xiao‑Liang Chen1
Estimated H-index: 1
(NCU: Nanchang University),
Jian‑Ping Fu1
Estimated H-index: 1
+ 3 AuthorsZhi‑Mou Tang1
Estimated H-index: 1
Abstract CXC195 exhibits strong protective effects against neuronal apoptosis by exerting antioxidant activity. However, the pharmacological function of CXC195 in cancer remains to be elucidated. The present study demonstrated that CXC195 exhibited significant cytotoxic effects, and induced cell cycle arrest and apoptosis in HepG2 human hepatocellular carcinoma (HCC) cell lines. Following treatment of HepG2 cells with 150 µΜ CXC195 for 24 , cell viability and the apoptotic rate were assessed usi...
Published on Dec 1, 2015in European Journal of Cell Biology3.02
Yongxin Yan4
Estimated H-index: 4
(Hebei Medical University),
Junxia Zhao4
Estimated H-index: 4
(Hebei Medical University)
+ 9 AuthorsJuan Zhao1
Estimated H-index: 1
(Hebei Medical University)
Abstract Tetramethylpyrazine (TMP) is an active compound extracted from the traditional Chinese medicinal herb Chuanxiong. Previously, we have shown that TMP induces human SH-SY5Y neuroblastoma cell differentiation toward the neuronal phenotype by targeting topoisomeraseIIβ (TopoIIβ), a protein implicated in neural development. In the present study, we aimed to elucidate whether the transcriptional factors specificity protein 1 (Sp1) and nuclear factor Y (NF-Y), in addition to the upstream signa...
Published on Aug 14, 2015in PLOS ONE2.78
Na Yu3
Estimated H-index: 3
(SYSU: Sun Yat-sen University),
Zhang Zhang6
Estimated H-index: 6
(SYSU: Sun Yat-sen University)
+ 10 AuthorsJian Ge30
Estimated H-index: 30
(SYSU: Sun Yat-sen University)
Background A traditional Chinese medicine, Tetramethylpyrazine (TMP), has been prescribed as a complementary treatment for glaucoma to improve patient prognosis. However, the pharmacological mechanism of action of TMP is poorly understood. In previous studies, we demonstrated that TMP exerts potent inhibitory effects on neovascularization, suppresses the tumorigenic behavior of glioma cells, and protects neural cells by regulating CXCR4 expression. Here, we further investigated whether the SDF-1...
Published on Jan 1, 2015in Neural Regeneration Research2.47
Haijun Gao6
Estimated H-index: 6
(JLU: Jilin University),
Pengfei Liu5
Estimated H-index: 5
(JLU: Jilin University)
+ 7 AuthorsHaiyan Huang3
Estimated H-index: 3
(JLU: Jilin University)
Ligustrazine (2,3,5,6-tetramethylpyrazine) is a major active ingredient of the Szechwan lovage rhizome and is extensively used in treatment of ischemic cerebrovascular disease. The mechanism of action of ligustrazine use against ischemic cerebrovascular diseases remains unclear at present. This study summarizes its protective effect, the optimum time window of administration, and the most effective mode of administration for clinical treatment of cerebral ischemia/reperfusion injury. We examine ...