In ovo injection of anti-chicken CD25 monoclonal antibodies depletes CD4+ CD25+ T cells in chickens

The CD4+ CD25+ cells have T regulatory cell properties in chickens. This study investigated the effect of in ovo injection of anti-chicken CD25 monoclonal antibodies (0.5 mg/egg) on CD4+ CD25+ cell depletion and on amounts of interleukin-2 mRNA and interferon-γ mRNA in CD4+ CD25− cells posthatch. Anti-chicken CD25 or PBS (control) was injected into 16-d-old embryos. Chicks hatched from eggs injected with anti-chicken CD25 antibodies had a lower...