Original paper
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
Abstract
CRISPR/Cas9 systems are a versatile tool for genome editing due to the highly efficient targeting of DNA sequences complementary to their RNA guide strands. However, it has been shown that RNA-guided Cas9 nuclease cleaves genomic DNA sequences containing mismatches to the guide strand. A better understanding of the CRISPR/Cas9 specificity is needed to minimize off-target cleavage in large mammalian genomes. Here we show that genomic sites could...
Paper Details
Title
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
Published Date
May 16, 2014
Journal
Volume
42
Issue
11
Pages
7473 - 7485
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Notes
History