Match!

Molecular analyses of dinosaur osteocytes support the presence of endogenous molecules.

Published on Jan 1, 2013in Bone4.36
· DOI :10.1016/j.bone.2012.10.010
Mary H. Schweitzer24
Estimated H-index: 24
(NCSU: North Carolina State University),
Wenxia Zheng10
Estimated H-index: 10
(NCSU: North Carolina State University)
+ 1 AuthorsMarshall W. Bern40
Estimated H-index: 40
(PARC)
Abstract
article i nfo The discovery of soft, transparent microstructures in dinosaur bone consistent in morphology with osteocytes was controversial. We hypothesize that, if original, these microstructures will have molecular features in com- mon with extant osteocytes. We present immunological and mass spectrometry evidence for preservation of proteins comprising extant osteocytes (Actin, Tubulin, PHEX, Histone H4) in osteocytes recovered from two non-avian dinosaurs. Furthermore, antibodies to DNA show localized binding to these microstructures, which also react positively with DNA intercalating stains propidium iodide (PI) and 4',6'-diamidino-2-phenylindole dihydrochloride (DAPI). Each antibody binds dinosaur cells in patterns similar to extant cells. These data are the first to support preservation of multiple proteins and to present multiple lines of evidence for material con- sistent with DNA in dinosaurs, supporting the hypothesis that these structures were part of the once living ani- mals. We propose mechanisms for preservation of cells and component molecules, and discuss implications for dinosaurian cellular biology.
  • References (83)
  • Citations (38)
References83
Newest
#1Karine Lalonde (Concordia University)H-Index: 5
#2Alfonso Mucci (McGill University)H-Index: 54
Last.Yves Gélinas (Concordia University)H-Index: 30
view all 4 authors...
#2Mary H. Schweitzer (MSU: Montana State University)H-Index: 24
Last.Joseph P. R. O. Orgel (IIT: Illinois Institute of Technology)H-Index: 21
view all 6 authors...
#1Johan Lindgren (Lund University)H-Index: 20
#2Per Uvdal (Lund University)H-Index: 21
Last.Desiree Douglas (Lund University)H-Index: 6
view all 12 authors...
#1Philipp Schneider (ETH Zurich)H-Index: 19
#2Matias Meier (ETH Zurich)H-Index: 3
Last.Ralph Müller (ETH Zurich)H-Index: 87
view all 4 authors...
#1Sarah L. Dallas (UMKC: University of Missouri–Kansas City)H-Index: 28
#2Lynda F. Bonewald (UMKC: University of Missouri–Kansas City)H-Index: 76
#1Dayong Guo (UMKC: University of Missouri–Kansas City)H-Index: 13
#2Lynda F. Bonewald (UMKC: University of Missouri–Kansas City)H-Index: 76
Cited By38
Newest
#1Victoria E. McCoy (University of Bonn)H-Index: 7
#2Sarah E. Gabbott (University of Leicester)H-Index: 21
Last.Xavier Delclòs (University of Barcelona)H-Index: 18
view all 11 authors...
#1Alida M. Bailleul (CAS: Chinese Academy of Sciences)H-Index: 6
#2Jingmai K. O'Connor (CAS: Chinese Academy of Sciences)H-Index: 22
Last.Mary H. SchweitzerH-Index: 24
view all 0 authors...
#1Dawid Surmik (University of Silesia in Katowice)H-Index: 3
#2Mateusz Dulski (University of Silesia in Katowice)H-Index: 12
Last.R. Pawlicki (Jagiellonian University Medical College)
view all 5 authors...
#1Valerie C. Wasinger (UNSW: University of New South Wales)H-Index: 23
#2Darren Curnoe (UNSW: University of New South Wales)H-Index: 19
Last.Paul Tacon (Griffith University)H-Index: 17
view all 10 authors...
#1Mary Higby Schweitzer (NCSU: North Carolina State University)H-Index: 3
#2Wenxia Zheng (NCSU: North Carolina State University)H-Index: 10
Last.Johan LindgrenH-Index: 20
view all 5 authors...
View next paperAnalyses of Soft Tissue from Tyrannosaurus rex Suggest the Presence of Protein