Indispensable structure of solution additives to prevent inactivation of lysozyme for heating and refolding

This article investigates solution additives that prevent misfolding of lysozyme from heating treatment and during refolding processes. Comparison of heat treatment of native lysozyme and oxidative refolding from the reduced and denatured state of lysozyme in the presence of 44 different additives revealed an indispensable chemical structure for the additives to be effective against heat‐induced misfolding and for refolding. The additives...