Original paper
A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome
Abstract
The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney and a B cell line. We used shotgun sequencing of transcripts to generate randomly distributed reads. Of these, 50% mapped to unique genomic locations, of which 80% corresponded to known exons. We found that 66% of the polyadenylated transcriptome mapped to known genes and...
Paper Details
Title
A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome
Published Date
Aug 15, 2008
Journal
Volume
321
Issue
5891
Pages
956 - 960
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Notes
History