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First evidence of avian metapneumovirus subtype A infection in turkeys in Egypt

Published on Aug 1, 2014in Tropical Animal Health and Production1.09
· DOI :10.1007/s11250-014-0591-8
Abdel-Azeem Sayed Abdel-Azeem1
Estimated H-index: 1
(FU: Fayoum University),
Giovanni Franzo10
Estimated H-index: 10
(UNIPD: University of Padua)
+ 5 AuthorsMattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
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Abstract
Although avian metapneumovirus (aMPV) infection has been reported in most regions of the world, to date, only subtype B has been detected in Egypt. At the end of November 2013, dry oropharyngeal swabs were collected during an outbreak of respiratory diseases in a free-range, multi-age turkey dealer farm in Northern Upper Egypt. The clinical signs that appeared when turkeys were 3 weeks-old were characterized by ocular and nasal discharge and swelling of sinuses. aMPV of subtype A was detected by real-time reverse transcription-polymerase chain reaction. In order to confirm the results and obtain more information on the molecular characteristics of the virus, F and G protein genes were partially sequenced and compared with previously published sequences deposited in GenBank by using BLAST. Subtype of the strain was confirmed by sequencing of partial F and G protein genes. The highest percentages of identity were observed when G sequence of the Egyptian strain was compared with the sequence of an aMPV-A isolated in Nigeria (96.4 %) and when the F sequence was compared with strains isolated respectively in Italy and in UK (97.1 %). Moreover, the alignment of the sequences with commercial subtype A vaccine or vaccine-derived strains showed differences in the Egyptian strain that indicate its probable field origin. The detection of aMPV in the investigated turkey flock highlights some relevant epidemiological issues regarding the role that multi-age farms and dealers may play in perpetuating aMPV infection within and among farms. To our knowledge, this is the first report of aMPV subtype A in Egypt.
  • References (17)
  • Citations (2)
Cite
References17
Newest
Published on Jun 1, 2014in Veterinary Research Communications0.98
Davide Giovanardi7
Estimated H-index: 7
,
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 3 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
The aim of this study was to evaluate if the exposure to Avian metapneumovirus (aMPV) and/or to Turkey hemorrhagic enteritis virus (THEV) was significant for the induction of episodes of colibacillosis in aMPV and THEV vaccinated turkeys. Colibacillosis-associated mortality was recorded and longitudinal virological studies performed in three consecutive turkey flocks reared in the same farm. aMPV and THEV diagnostic swabs and blood samples were made once a week up to 14 weeks of age. Swabs were ...
Published on May 4, 2014in Avian Pathology1.96
José Francisco Rivera-Benitez5
Estimated H-index: 5
(UNAM: National Autonomous University of Mexico),
Rebeca Martínez-Bautista2
Estimated H-index: 2
(UNAM: National Autonomous University of Mexico)
+ 1 AuthorsHumberto Ramírez-Mendoza8
Estimated H-index: 8
(UNAM: National Autonomous University of Mexico)
We conducted a longitudinal study to detect and isolate avian metapneumovirus (aMPV) in two highly productive poultry areas in Mexico. A total of 968 breeder hens and pullets from 2 to 73 weeks of age were analysed. Serology was performed to detect aMPV antibodies and 105 samples of tracheal tissue were collected, pooled by age, and used for attempted virus isolation and aMPV nested reverse transcriptase-polymerase chain reaction (nRT-PCR). The serological analysis indicated that 100% of the sam...
Published on Jan 2, 2014in Avian Pathology1.96
V. Listorti4
Estimated H-index: 4
(UNIBO: University of Bologna),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 5 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
Live vaccines predominantly control avian metapneumovirus (aMPV) infection in poultry flocks, but vaccine virus can be found for extended periods after application. The most frequently used aMPV vaccine in Italy, VCO3 subtype B, was shown to contain a unique Tru9I restriction endonuclease site within the amplicons produced by a commonly used aMPV diagnostic reverse transcriptase (RT)-nested polymerase chain reaction (PCR). Analysis of European and database logged subtype B aMPV sequences confirm...
Published on Jul 1, 2013in Emerging Infectious Diseases7.18
Li Wei8
Estimated H-index: 8
,
Shanshan Zhu7
Estimated H-index: 7
+ 7 AuthorsJue Liu12
Estimated H-index: 12
Avian metapneumovirus causes acute respiratory tract infection and reductions in egg production in various avian species. We isolated and characterized an increasingly prevalent avian metapneumovirus subgroup C strain from meat-type commercial chickens with severe respiratory signs in China. Culling of infected flocks could lead to economic consequences.
Published on Jun 1, 2013in Avian Pathology1.96
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 4 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
In recent years, special attention has been paid to real-time polymerase chain reaction (PCR) for avian metapneumovirus (AMPV) diagnosis, due to its numerous advantages over classical PCR. A new multiplex quantitative real-time reverse transcription-PCR (qRT-PCR) with molecular beacon probe assay, designed to target the SH gene, was developed. The test was evaluated in terms of specificity, sensitivity and repeatability, and compared with conventional RT nested-PCR based on the G gene. All of th...
Published on Jan 1, 2013in Large Animal Review0.26
Cecchinato Mattia6
Estimated H-index: 6
,
Michele Drigo14
Estimated H-index: 14
+ 7 AuthorsE. Catelli12
Estimated H-index: 12
Published on Dec 1, 2012in Avian Diseases1.31
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua),
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna)
+ 4 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
SUMMARY. The current information on the prevalence of avian metapneumovirus (aMPV) infection in layers is fragmentary and its true impact on egg production often remains unknown or unclear. In order to draw an epidemiologic picture of aMPV presence in layer flocks in Italy, a survey was performed on 19 flocks of pullets and layers based on longitudinal studies or sporadic samplings. aMPV was detected by reverse transcription (RT)-PCR, and blood samples were collected for serology by aMPV ELISA. ...
Published on Oct 1, 2011in Avian Pathology1.96
Caterina Lupini8
Estimated H-index: 8
(UNIBO: University of Bologna),
Mattia Cecchinato12
Estimated H-index: 12
(UNIPD: University of Padua)
+ 2 AuthorsE. Catelli12
Estimated H-index: 12
(UNIBO: University of Bologna)
Avian metapneumovirus (aMPV) subtype A was isolated from 7-week-old turkeys showing respiratory disease typical of turkey rhinotracheitis. Comparison of the virus sequence with previously determined vaccine marker sequences showed that the virulent virus had originated from a licensed live subtype A aMPV vaccine. The vaccine had neither been in use on the farm within a period of at least 6 months nor had it been used on farms within a distance of approximately 5 km. Isolation of the virus and ex...
Published on Mar 1, 2011in Avian Diseases1.31
Jorge Luis Chacón10
Estimated H-index: 10
(USP: University of São Paulo),
Matheus Y. Mizuma2
Estimated H-index: 2
(USP: University of São Paulo)
+ 5 AuthorsAntonio José Piantino Ferreira16
Estimated H-index: 16
(USP: University of São Paulo)
SUMMARY. Avian metapneumovirus (AMPV) causes turkey rhinotracheitis and is associated with swollen head syndrome in chickens, which is usually accompanied by secondary infections that increase mortality. AMPVs circulating in Brazilian vaccinated and nonvaccinated commercial chicken and turkey farms were detected using a universal reverse transcriptase (RT)-PCR assay that can detect the four recognized subtypes of AMPV. The AMPV status of 228 farms with respiratory and reproductive disturbances w...
Published on Jul 1, 2010in Nucleic Acids Research11.15
Federico Abascal19
Estimated H-index: 19
(CSIC: Spanish National Research Council),
Rafael Zardoya54
Estimated H-index: 54
(CSIC: Spanish National Research Council),
Maximilian J. Telford1
Estimated H-index: 1
(CSIC: Spanish National Research Council)
We present TranslatorX, a web server designed to align protein-coding nucleotide sequences based on their corresponding amino acid translations. Many comparisons between biological sequences (nucleic acids and proteins) involve the construction of multiple alignments. Alignments represent a statement regarding the homology between individual nucleotides or amino acids within homologous genes. As protein-coding DNA sequences evolve as triplets of nucleotides (codons) and it is known that sequence...
Cited By2
Newest
Published on Jan 1, 2019in Veterinary Microbiology2.79
Mengmeng Yu1
Estimated H-index: 1
(Harbin Veterinary Research Institute),
Lixiao Xing1
Estimated H-index: 1
(Harbin Veterinary Research Institute)
+ 18 AuthorsMuhammad Farooque (Harbin Veterinary Research Institute)
Abstract Avian metapneumovirus (aMPV), which has been reported in many countries, causes an acute upper respiratory tract disease in chickens and turkeys. Although aMPV was first detected in China in 1999, there has been no further effort to isolate and characterize the aMPV subtype B (aMPV/B) from field outbreaks. In the present study, we used Vero cells to culture a viral strain, LN16, isolated from chickens with swollen head syndrome. The results of RT-PCR, indirect immunofluorescent antibody...
Published on Aug 27, 2018in Avian Diseases1.31
E. Bayraktar (CEVA Logistics), S. Umar (Istanbul University)+ 7 AuthorsHuseyin Yilmaz13
Estimated H-index: 13
(Istanbul University)
Viral respiratory diseases including avian metapneumovirus (aMPV) have a significant economic impact on poultry industries. The frequency and genotype diversity of aMPV in Turkish broiler flocks is not known at present. The aim of this study was to report the first molecular identification and phylogeny of aMPV which is circulating in Turkish broiler flocks. Trachea tissue samples and tracheal swabs were collected from 110 broiler flocks distributed in different geographical regions in Turkey be...
Published on Sep 1, 2017in Avian Diseases1.31
Claudia Maria Tucciarone5
Estimated H-index: 5
,
Marianna Andreopoulou + 3 AuthorsMattia Cecchinato12
Estimated H-index: 12
SUMMARY Avian metapneumovirus (aMPV) is considered a major pathogen for turkeys but its impact on chicken production is still partially neglected, even though it is fully acknowledged as a primary pathogen in chickens as well. The lack of structured diagnostic surveys does not allow a pervasive understanding of aMPV epidemiology. Being that aMPV is almost an everyday challenge for farmers and veterinarians, a more accurate report of its presence should be detailed, posing the basis for a deep an...
View next paperFirst Molecular Characterization of Avian Metapneumovirus (aMPV) in Turkish Broiler Flocks