Original paper
LCP-FRAP Assay for Pre-Screening Membrane Proteins for In Meso Crystallization
Abstract
Fluorescence recovery after photobleaching was used to study the diffusion of two integral membrane proteins, bacteriorhodopsin and beta2-adrenergic receptor, in lipidic cubic phase (LCP). We found that the diffusion properties within the LCP matrix strongly depend on the protein construct and applied screening conditions. Common precipitants often induce restriction on diffusion of proteins in LCP and thereby impede their chances for...
Paper Details
Title
LCP-FRAP Assay for Pre-Screening Membrane Proteins for In Meso Crystallization
Published Date
Nov 7, 2008
Journal
Volume
8
Issue
12
Pages
4307 - 4315
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Notes
History