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Somatic embryogenesis, plant regeneration, and cryopreservation for Torreya taxifolia, a highly endangered coniferous species

Published on Jun 1, 2012in In Vitro Cellular & Developmental Biology – Plant1.45
· DOI :10.1007/s11627-012-9433-4
X. Ma1
Estimated H-index: 1
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
+ 2 AuthorsGerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology)
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Abstract
Torreya taxifolia Arn., an ancient evergreen tree, is on the brink of extinction from attack by a fungal disease, recently reported to be caused by a novel isolate of Fusarium. We report the development of a somatic embryogenesis tissue culture system that can be used for cryogenic storage of T. taxifolia cultures and subsequent plant regeneration. Initiation of embryogenic tissue from immature zygotic embryos occurred on a conifer tissue culture medium containing 0.25 % activated charcoal, 43.8 mM maltose, 0.5 mM 2,4-dichlorophenoxacetic acid, 0.2 mM 6-benzylaminopurine, 0.2 mM kinetin, 0.1 μM brassinolide, 3.8 μM abscisic acid, 20.5 μM biotin, 1.13 μM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Embryo induction ranged from 60 to 100 % across six seed sources. Somatic embryo development occurred on a medium containing 43.8 mM maltose, 1 % activated charcoal, 37.8 μM abscisic acid, 20.5 μM biotin, 0.1 μM brassinolide, 0.205 mM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Germination of somatic embryos ranged from 64 to 82 %. Embryogenic tissue cultures from 30 genotypes representing seed from six mother trees were cryopreserved, and culture recovery was demonstrated after freezing. In contrast to many other coniferous tree seeds, the measured water potential (−MPa) of T. taxifolia megagametophyte tissue rose greatly during seed after-ripening. Duplication of this rise in vitro allowed development of somatic embryos to the cotyledonary stage.
  • References (34)
  • Citations (19)
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References34
Newest
Published on Apr 19, 2017
Aljos Farjon1
Estimated H-index: 1
This revised and updated handbook includes detailed information on all 615 conifers, temperate as well as tropical, this encyclopedic work offers users as diverse as ecologists, gardeners, foresters and conservationists the accumulated knowledge of these trees obtained in 35 years of academic research, presented in an easily searchable format.
Published on Jun 1, 2011in Plant Disease3.58
Jason A. Smith21
Estimated H-index: 21
,
Kerry O'Donnell59
Estimated H-index: 59
+ 6 AuthorsRon Determann1
Estimated H-index: 1
Smith, J. A., O’Donnell, K., Mount, L. L., Shin, K., Peacock, K., Trulock, A., Spector, T., Cruse-Sanders, J., and Determann, R. 2011. A novel Fusarium species causes a canker disease of the critically endangered conifer, Torreya taxifolia. Plant Dis. 95:633-639. A canker disease of Florida torreya (Torreya taxifolia) has been implicated in the decline of this critically endangered species in its native range of northern Florida and southeastern Georgia. In surveys of eight Florida torreya sites...
Published on Jan 1, 2011in Methods of Molecular Biology
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
Published on May 3, 2010
Aljos Farjon1
Estimated H-index: 1
Foreword Preface The conifers of the world, an introduction The distribution and ecology of conifers The economic importance of conifers The conservation of conifer diversity Synopsis of families and genera Taxonomic treatment of families, with keys to families Taxonomic treatment of genera and species (in alphabetical order), with keys to sections, subsections and species Glossary References Lists of illustrations Index to botanical names of conifers
Published on Apr 27, 2010
Aljos Farjon1
Estimated H-index: 1
The first handbook to include detailed information on all 615 conifers, temperate as well as tropical, this encyclopedic work offers users as diverse as ecologists, gardeners, foresters and conservationists the accumulated knowledge of these trees obtained in 30 years of academic research, presented in an easily searchable format.
Published on Jan 1, 2010in Biological Conservation4.45
Pati Vitt12
Estimated H-index: 12
,
Kayri Havens19
Estimated H-index: 19
+ 2 AuthorsEmily Yates1
Estimated H-index: 1
Abstract Rapid climate change has the potential to alter the location of bioclimatic envelopes for a significant portion of the world’s flora. Plant species will respond variously via phenotypic plasticity, evolutionary adaptation, migration, or extinction. When fragmentation limits migration potential of many species or when natural migration rates are outstripped by the pace of climate change, some propose purposeful, human-mediated migration (assisted migration) as a solution. Here, we join t...
Published on Jun 1, 2009in Tree Physiology3.48
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
Shannon Johnson8
Estimated H-index: 8
(Georgia Institute of Technology)
Summary Stage-specific measurements of female gametophyte (FG) and embryo pH (hydrogen ion concentration) were made through the sequence of loblolly pine (Pinus taeda L.) seed development. The FG tissue from two open-pollinated trees showed similar pH profiles starting at 5.5 shortly after fertilization, increasing to about 6.1 at stage 7, levelling off at 6.3–6.5 towards the end of development and dropping to 6.0 just before cone opening. Measurements of the chalazal end were 0.05–0.2 pH units ...
Published on Jun 1, 2009in Tree Physiology3.48
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
Shannon Johnson8
Estimated H-index: 8
(Georgia Institute of Technology)
Summary Water potential (W) and osmotic potential (Ws) were measured weekly through the sequence of seed development in megagametophytes of loblolly pine (Pinus taeda L.). A Wescor 5500XRS vapor pressure osmometer, modified with a cycle hold switch, was used to measure W for whole megagametophytes containing embryos. The W measurements for megagametophytes with embryos removed were also attempted but readings were distorted due to cell lysates from the cut surfaces. Six seasonal sets of megagame...
Published on Jan 1, 2009in Plant Cell Tissue and Organ Culture2.20
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
Shannon Johnson8
Estimated H-index: 8
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
Somatic embryogenesis (SE) is expected to play an important role in the future of US forests by providing increased productivity, sustainability, and uniformity. For broad scale implementation to occur, SE technology must work with a variety of genetically diverse trees. Douglas fir (Pseudotsuga menziesii (Mirb) Franco) is the dominant tree in the Pacific Northwest and has great economic and recreational value. We have developed a highly effective medium for initiation of embryogenic tissue of D...
Mukul Manjari Datta2
Estimated H-index: 2
(CU: University of Calcutta),
Sumita Jha20
Estimated H-index: 20
(CU: University of Calcutta)
We describe an efficient process for regeneration of Taxus wallichiana (Zucc) plants from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength Lloyd and McCown’s basal medium supplemented with SH vitamin (1/2 WPMSH), 0.5 mg I−1 6-benzyladenine (BA) and 1.0–2.0 mg I−1 a-Napthaleneacetic acid (NAA) produced compact yellow (CY) callus after 4 weeks of culture. The 8-week-old CY call! (lines CY-A and CY-B) were initially slow growing but proliferated on transfer to...
Cited By19
Newest
Published on May 20, 2019in In Vitro Cellular & Developmental Biology – Plant1.45
Chang Ho Ahn , Kweon Heo10
Estimated H-index: 10
(Kangwon National University)
+ 1 AuthorsYong Eui Choi
Korean arbor vitae (KAV; Thuja koraiensis Nakai) is a critically endangered coniferous tree in Korea. Here, we report the somatic embryogenesis (SE) and cryopreservation system that can be used for micropropagation of KAV and long-term storage of KAV cultures. To induce SE in KAV, the influence of the developmental stage of zygotic embryos and the effect of basal medium on embryogenesis induction were examined. The developmental stage of zygotic embryos had a significant effect on the embryogene...
Published on Feb 1, 2018in In Vitro Cellular & Developmental Biology – Plant1.45
Ahmed Salama (U of G: University of Guelph), Elena Popova3
Estimated H-index: 3
(U of G: University of Guelph)
+ 3 AuthorsPraveen K. Saxena41
Estimated H-index: 41
(U of G: University of Guelph)
In this study conservation of Castilleja levisecta Greenm., a globally endangered species was addressed through in vitro cryopreservation of shoot tips. In vitro cultures were successfully established using seedlings received from British Columbia, Canada. Shoot tips excised from in vitro propagated plants were cryopreserved using a droplet-vitrification method following optimization of individual protocol steps such as pre-culture, treatment with vitrification solutions, and unloading. The high...
Published on Jan 1, 2018
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
John Frampton7
Estimated H-index: 7
Fraser fir ( Abies fraseri [Pursh] Poir.) is a coniferous species native to the Southern Appalachian Mountains of the Eastern United States. Fraser fir has high economic and recreational value but is vulnerable to extinction due to introduced pests and global warming. Somatic embryogenesis may assist in the clonal production of Christmas trees and conservation of rare and valuable germplasm via cryopreservation. Improved protocols for embryogenic tissue initiation, culture capture and growth, so...
Published on Jan 1, 2018
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology)
Somatic embryogenesis (SE) technology has the potential to be the lowest-cost method to rapidly produce large numbers of high-value seedlings with desired characteristics for plantation forestry. SE is expected to play an important role in the future to increase forest productivity, sustainability and uniformity. SE technology has the advantages of: (1) shortening time to produce desired Planting stock, (2) allowing control of genetic variation, (3) permitting commercial hybrids, and (4) facilit...
Published on Oct 1, 2017in Industrial Crops and Products4.19
Sandra Nunes3
Estimated H-index: 3
,
Liliana Marum8
Estimated H-index: 8
+ 4 AuthorsConceição Santos32
Estimated H-index: 32
(University of Porto)
Abstract Clonal breeding programs of the highly important interspecific hybrid Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis require the establishment of robust cryopreservation protocols, with no genetic variability. Embryonal masses of this hybrid were successfully cryopreserved by a fast slow-freezing method. The protocol was optimized by testing the effects of dimethyl sulfoxide (DMSO) based cryoprotectant solutions, sugar cryoprotectants (e.g. sucrose or maltose, 0.4 M)...
Published on Jan 1, 2017in Botanical Research
本文研究了生根培养基中添加不同浓度的油菜素内酯(BR)对桔梗组培幼苗生长和生理特性的影响,外源BR的浓度为0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L。结果表明:幼苗不定根的条数、幼苗节数、叶绿体色素含量、POD和SOD活性均随BR浓度的增加呈先增高后降低趋势,在0.10 mg/L处理达到最大值,说明该处理有利于培养健壮幼苗,提高叶绿素含量和增强幼苗的抗性。 In this study, the effects of Brassinosteriod (BR) on growth and physiological properties of Platycodon grandiflorum seedlings were conducted by adding exogenous BR in the root inducing medium, and the concentration of exogenous BR is 0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L. The results showed that the nu...
Published on May 1, 2016in New Forests1.60
Gerald S. Pullman27
Estimated H-index: 27
(Georgia Institute of Technology),
Katie Olson1
Estimated H-index: 1
(Georgia Institute of Technology)
+ 3 AuthorsKylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
Fraser fir (Abies fraseri [Pursh] Poir.) is a coniferous species native to the Southern Appalachian Mountains of the eastern United States. The species has high economic and recreational value but is vulnerable to extinction due to introduced pests and global warming. Somatic embryogenesis technology may assist in the clonal production of desired lines of Christmas trees and safekeeping of rare and valuable germplasm via cryopreservation. We have developed a highly effective medium for initiatio...
Published on Mar 1, 2016in Protoplasma2.63
Neusa Steiner12
Estimated H-index: 12
(UFSC: Universidade Federal de Santa Catarina),
Francine L. Farias-Soares5
Estimated H-index: 5
(UFSC: Universidade Federal de Santa Catarina)
+ 7 AuthorsMiguel Pedro Guerra28
Estimated H-index: 28
(UFSC: Universidade Federal de Santa Catarina)
Somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the Brazilian pine (Araucaria angustifolia). In the present study, we investigated the morphological and ultrastructural organization of A. angustifolia somatic embryo development by means of optical and electron microscopy. The proembryogenic stage was characterized by the proliferation of proembryogenic masses (PEMs), which are cell...
Published on Jan 1, 2016
Dariusz Kulus4
Estimated H-index: 4
(University of Technology and Life Sciences in Bydgoszcz)
Cryopreservation is the most safe long-term conservation method of non-orthodox seed species. Somatic embryogenesis, on the other hand, is considered to be the most efficient (micro)propagation technique. By combining in vitro tissue culture techniques with cryoconservation, it is possible to develop highly diverse gene banks on a small surface at reduced costs from cutting down on labor and laboratory consumables. The application of embryonic tissue for storage in liquid nitrogen is very benefi...
Published on Jan 1, 2016in Methods of Molecular Biology
Krystyna Klimaszewska21
Estimated H-index: 21
,
Catherine Hargreaves2
Estimated H-index: 2
(Scion)
+ 1 AuthorsJean-François Trontin14
Estimated H-index: 14
This review compiles research results published over the last 14 years on conifer somatic embryogenesis (SE). Emphasis is placed on the newest findings that affect the response of seed embryos (typical explants) and shoot primordia (rare explants) to the induction of SE and long-term culture of early somatic embryos. Much research in recent years has focused on maturation of somatic embryos, with respect to both yield and quality, as an important stage for the production of a large number of vig...