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Abstract
Torreya taxifolia Arn., an ancient evergreen tree, is on the brink of extinction from attack by a fungal disease, recently reported to be caused by a novel isolate of Fusarium. We report the development of a somatic embryogenesis tissue culture system that can be used for cryogenic storage of T. taxifolia cultures and subsequent plant regeneration. Initiation of embryogenic tissue from immature zygotic embryos occurred on a conifer tissue culture medium containing 0.25 % activated charcoal, 43.8 mM maltose, 0.5 mM 2,4-dichlorophenoxacetic acid, 0.2 mM 6-benzylaminopurine, 0.2 mM kinetin, 0.1 μM brassinolide, 3.8 μM abscisic acid, 20.5 μM biotin, 1.13 μM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Embryo induction ranged from 60 to 100 % across six seed sources. Somatic embryo development occurred on a medium containing 43.8 mM maltose, 1 % activated charcoal, 37.8 μM abscisic acid, 20.5 μM biotin, 0.1 μM brassinolide, 0.205 mM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Germination of somatic embryos ranged from 64 to 82 %. Embryogenic tissue cultures from 30 genotypes representing seed from six mother trees were cryopreserved, and culture recovery was demonstrated after freezing. In contrast to many other coniferous tree seeds, the measured water potential (−MPa) of T. taxifolia megagametophyte tissue rose greatly during seed after-ripening. Duplication of this rise in vitro allowed development of somatic embryos to the cotyledonary stage.
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References34
Michael R. Becwar2
Estimated H-index: 2
,
Gerald S. Pullman1
Estimated H-index: 1
Somatic embryogenesis, the formation and development of embryos from somatic (vegetative) tissues under in vitro conditions, was first discovered in carrot (Steward et al., 1958) and in conifers with Picea abies in 1985 (Hakman et al., 1985). The first report of somatic embryogenesis with Pinus taeda was in 1987 (Gupta & Durzan, 1987).
Ref 36Cited 84 Source Cite this paper
Claudio Stasolla28
Estimated H-index: 28
(University of Winnipeg),
Edward C. Yeung40
Estimated H-index: 40
(University of Calgary)
Somatic embryogenesis of coniferous species was first reported more than 20 years ago. Since then, there has been an explosion of research aimed at developing and optimizing protocols for efficient regeneration of plantlets. Although routinely used both as a means of propagation, as well as a valuable model system for investigating the structural, physiological, and molecular events occurring duri...
Ref 104Cited 177 Source Cite this paper
Pramod K. Gupta11
Estimated H-index: 11
("University of California, Berkeley"),
D. J. Durzan33
Estimated H-index: 33
("University of California, Berkeley")
Loblolly pine (Pinus taeda L.) is the leading commercial timber species in the southern United States. Carolus Linnaeus gave loblolly pine its scientific name, Pinus taeda, over 225 years ago. Taeda is the ancient name for resinous pines. It comes from the Latin, meaning torch. In Roman times, taeda was applied to several of the hard, pitchy pines (Wahlenberg 1960).
Ref 23Cited 100 Download Pdf Cite this paper
Nanfei Xu7
Estimated H-index: 7
,
Barbara Johns4
Estimated H-index: 4
,
Gerald S. Pullman18
Estimated H-index: 18
(Weyerhaeuser)
... (1 others)
Performing RNA differential display analysis on small tissue samples is difficult since much RNA, the initial template for the reaction, is lost during conventional isolation procedures. We have developed a rapid method which employs oligo-dT beads to capture mRNA from cell lysates. Subsequent reactions are primed directly from the beads, thus RT and PCR reactions can be completed within a few hou...
Ref 15Cited 23 Source Cite this paper
Aljos Farjon1
Estimated H-index: 1
Foreword Preface The conifers of the world, an introduction The distribution and ecology of conifers The economic importance of conifers The conservation of conifer diversity Synopsis of families and genera Taxonomic treatment of families, with keys to families Taxonomic treatment of genera and species (in alphabetical order), with keys to sections, subsections and species Glossary References List...
Cited 97 Source
2016 in Plant Cell Reports [IF: 2.87]
Gerald S. Pullman20
Estimated H-index: 20
(Georgia Institute of Technology),
Shannon Johnson8
Estimated H-index: 8
(Georgia Institute of Technology),
Gary F. Peter25
Estimated H-index: 25
(University of Florida)
... (2 others)
Clonal production of loblolly pine (Pinus taeda L.) through somatic embryogenesis has the potential to meet the increasing industrial demands for high-quality uniform raw materials. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Twenty-five newly initiated loblolly pine genotypes were followed through the process of liquid culture establish...
Ref 26Cited 90 Download Pdf Cite this paper
John W.G. Cairney45
Estimated H-index: 45
(University of Western Sydney),
Nanfei Xu7
Estimated H-index: 7
,
Gerald S. Pullman18
Estimated H-index: 18
(Weyerhaeuser)
... (2 others)
In production biological systems, monitoring and controlling the growth environment is possible, but assessing the metabolic competency of the organism is more difficult. Somatic embryogenesis (SE), a tissue-culture method for multiplying embryos asexually, has great potential to capture at low cost the genetic gain from breeding and genetic-engineering programs. Loblolly pine, however, has proven...
Ref 20Cited 16 Download Pdf Cite this paper
2016 in Plant Cell Reports [IF: 2.87]
Paula P. Chee1
Estimated H-index: 1
Taxusbrevifolia is the source of paclitaxel (Taxol®), an anticancer drug. A method for regeneration ofTaxus brevifolia from immature zygotic embryos via somatic embryogenesis is described. Embryogenic callus tissues were obtained by culturing immature zygotic embryos on Lloyd and McCown medium (MCM) supplemented with 160 μM 2,4-dichlorophenoxyacetic acid (2,4-D) + 5 μM benzylaminopurine (BA) + 5 μ...
Ref 16Cited 18 Download Pdf Cite this paper
2016 in Plant Cell Reports [IF: 2.87]
Gerald S. Pullman18
Estimated H-index: 18
(Weyerhaeuser),
Pramod K. Gupta16
Estimated H-index: 16
(Weyerhaeuser),
Roger Timmis14
Estimated H-index: 14
(Weyerhaeuser)
... (3 others)
The combination of abscisic acid (ABA) and activated carbon increased Norway spruce (Picea abies L., Karst.) cotyledonary somatic embryo yields, increased the number of genotypes forming cotyledonary embryos, caused embryos to form that exhibited improved maturation characteristics, and reduced embryo production costs. Somatic embryos increased in size, showed larger apical regions, became more zy...
Ref 37Cited 42 Download Pdf Cite this paper
2016 in Plant Cell Reports [IF: 2.87]
G. S. Pullman1
Estimated H-index: 1
,
Y. Zhang1
Estimated H-index: 1
,
Bao H. Phan4
Estimated H-index: 4
(University of Georgia)
Somatic embryogenesis (SE), the most promising technology for the large-scale production of high-value coniferous trees from advanced breeding and genetic engineering programs, is expected to play an important role in increasing productivity, sustainability, and the uniformity of future U.S. forests. To be successful for commercial use, SE technology must work with a variety of genetically diverse...
Ref 22Cited 72 Download Pdf Cite this paper
Cited by17
2016 in Journal of Proteomics [IF: 3.91]
André Luis Wendt dos Santos5
Estimated H-index: 5
(University of São Paulo),
Paula Elbl4
Estimated H-index: 4
(University of São Paulo),
Bruno Viana Navarro3
Estimated H-index: 3
(University of São Paulo)
... (4 others)
GeLC–MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angustifolia RNA-Seq data, 2398 non-redundant proteins were identified. The ...
Ref 100Cited 8 Source Cite this paper
2016 in New Forests [IF: 1.54]
Gerald S. Pullman20
Estimated H-index: 20
(Georgia Institute of Technology),
Kylie Bucalo8
Estimated H-index: 8
(Georgia Institute of Technology)
The shift from vegetative to embryogenic growth requires tissue to enter a radically different program of development and can be studied in vitro through the development of somatic embryos. From an applied perspective somatic embryogenesis (SE) is expected to play an important role in increasing productivity, sustainability, and uniformity of future forests. For commercial use, SE technology must ...
Ref 157Cited 18 Download Pdf Cite this paper
Ahmed Salama (University of Guelph), Elena Popova5
Estimated H-index: 5
(University of Guelph),
Maxwell P. Jones2
Estimated H-index: 2
(University of Guelph)
... (3 others)
In this study conservation of Castilleja levisecta Greenm., a globally endangered species was addressed through in vitro cryopreservation of shoot tips. In vitro cultures were successfully established using seedlings received from British Columbia, Canada. Shoot tips excised from in vitro propagated plants were cryopreserved using a droplet-vitrification method following optimization of individual...
Ref 39 Download Pdf Cite this paper
Qixiang Zhang1
Estimated H-index: 1
(Agriculture and Forestry University),
Weibo Xiang (Agriculture and Forestry University), Jin Yao (Agriculture and Forestry University) ... (6 others)
Key message This paper provides an important direct proembryogenic mass initiation and plant regeneration method that might be supportive and stable for gene transformation and commercial production of Torreya grandis. Abstract Torreya grandis is an important and commer- cially valuable tree that is utilized as a source of essential oil and nuts. Propagation researches of T. grandis have been focu...
Ref 28 Download Pdf Cite this paper
2016 in Acta Physiologiae Plantarum [IF: 1.36]
Sharrmila Rengeswari Palanyandy3
Estimated H-index: 3
(Universiti Putra Malaysia),
Periasamy Suranthran5
Estimated H-index: 5
(Universiti Putra Malaysia),
Saikat Gantait15
Estimated H-index: 15
(Universiti Putra Malaysia)
... (5 others)
In the present study, we report the in vitro development of polyembryoids with identification of a definite stage that can be used for subsequent uniform plantlet regeneration in oil palm (Elaeis guineensis Jacq.). Induction and maturation of polyembryoids was accomplished when cell suspension culture was transferred in MS (Murashige and Skoog, Physiol Plant 15:473–497, 1962) semisolid medium cons...
Ref 23Cited 9 Download Pdf Cite this paper
Somatic embryogenesis (SE) technology has the potential to be the lowest-cost method to rapidly produce large numbers of high-value seedlings with desired characteristics for plantation forestry. SE is expected to play an important role in the future to increase forest productivity, sustainability and uniformity. SE technology has the advantages of: (1) shortening time to produce desired Planting ...
Ref 92 Source Cite this paper