Somatic embryogenesis, plant regeneration, and cryopreservation for Torreya taxifolia, a highly endangered coniferous species

Published on Jun 1, 2012in In Vitro Cellular & Developmental Biology – Plant 1.06
· DOI :10.1007/s11627-012-9433-4
X. Ma1
Estimated H-index: 1
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
+ 2 AuthorsGerald S. Pullman25
Estimated H-index: 25
(Georgia Institute of Technology)
Abstract
Torreya taxifolia Arn., an ancient evergreen tree, is on the brink of extinction from attack by a fungal disease, recently reported to be caused by a novel isolate of Fusarium. We report the development of a somatic embryogenesis tissue culture system that can be used for cryogenic storage of T. taxifolia cultures and subsequent plant regeneration. Initiation of embryogenic tissue from immature zygotic embryos occurred on a conifer tissue culture medium containing 0.25 % activated charcoal, 43.8 mM maltose, 0.5 mM 2,4-dichlorophenoxacetic acid, 0.2 mM 6-benzylaminopurine, 0.2 mM kinetin, 0.1 μM brassinolide, 3.8 μM abscisic acid, 20.5 μM biotin, 1.13 μM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Embryo induction ranged from 60 to 100 % across six seed sources. Somatic embryo development occurred on a medium containing 43.8 mM maltose, 1 % activated charcoal, 37.8 μM abscisic acid, 20.5 μM biotin, 0.1 μM brassinolide, 0.205 mM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Germination of somatic embryos ranged from 64 to 82 %. Embryogenic tissue cultures from 30 genotypes representing seed from six mother trees were cryopreserved, and culture recovery was demonstrated after freezing. In contrast to many other coniferous tree seeds, the measured water potential (−MPa) of T. taxifolia megagametophyte tissue rose greatly during seed after-ripening. Duplication of this rise in vitro allowed development of somatic embryos to the cotyledonary stage.
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  • Citations (18)
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References34
Published on Jan 1, 2003in Plant Cell Tissue and Organ Culture 2.00
Claudio Stasolla14
Estimated H-index: 14
(University of Winnipeg),
Edward C. Yeung40
Estimated H-index: 40
(University of Calgary)
Somatic embryogenesis of coniferous species was first reported more than 20 years ago. Since then, there has been an explosion of research aimed at developing and optimizing protocols for efficient regeneration of plantlets. Although routinely used both as a means of propagation, as well as a valuable model system for investigating the structural, physiological, and molecular events occurring during embryo development, in vitro embryogenesis is still problematic for some coniferous species. Majo...
186 Citations Source Cite
Published on Dec 1, 1997in Plant Molecular Biology Reporter 1.84
Nanfei Xu5
Estimated H-index: 5
,
Barbara Johns2
Estimated H-index: 2
+ 1 AuthorsJohn Cairney12
Estimated H-index: 12
Performing RNA differential display analysis on small tissue samples is difficult since much RNA, the initial template for the reaction, is lost during conventional isolation procedures. We have developed a rapid method which employs oligo-dT beads to capture mRNA from cell lysates. Subsequent reactions are primed directly from the beads, thus RT and PCR reactions can be completed within a few hours of tissue harvest. This approach allows us to perform differential display on a single pine embry...
25 Citations Source Cite
Published on May 3, 2010
Aljos Farjon1
Estimated H-index: 1
Foreword Preface The conifers of the world, an introduction The distribution and ecology of conifers The economic importance of conifers The conservation of conifer diversity Synopsis of families and genera Taxonomic treatment of families, with keys to families Taxonomic treatment of genera and species (in alphabetical order), with keys to sections, subsections and species Glossary References Lists of illustrations Index to botanical names of conifers
110 Citations
Published on Apr 1, 2003in Plant Cell Reports 2.99
Gerald S. Pullman25
Estimated H-index: 25
,
Shannon Johnson7
Estimated H-index: 7
+ 2 AuthorsNanfei Xu5
Estimated H-index: 5
(Monsanto)
Clonal production of loblolly pine (Pinus taeda L.) through somatic embryogenesis has the potential to meet the increasing industrial demands for high-quality uniform raw materials. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Twenty-five newly initiated loblolly pine genotypes were followed through the process of liquid culture establishment, embryo maturation, germination, and retrieval from cryogenic storage. A maturation medium, cap...
95 Citations Source Cite
Published on Dec 1, 1996in Plant Cell Reports 2.99
Paula P. Chee1
Estimated H-index: 1
Taxusbrevifolia is the source of paclitaxel (Taxol®), an anticancer drug. A method for regeneration ofTaxus brevifolia from immature zygotic embryos via somatic embryogenesis is described. Embryogenic callus tissues were obtained by culturing immature zygotic embryos on Lloyd and McCown medium (MCM) supplemented with 160 μM 2,4-dichlorophenoxyacetic acid (2,4-D) + 5 μM benzylaminopurine (BA) + 5 μM naphthaleneacetic acid (NAA) for 4 weeks. Putative embryoids were obtained following transfer of c...
18 Citations Source Cite
Published on Jul 1, 2005in Plant Cell Reports 2.99
Gerald S. Pullman25
Estimated H-index: 25
(Weyerhaeuser),
Pramod K. Gupta15
Estimated H-index: 15
(Weyerhaeuser)
+ 3 AuthorsE. Welty1
Estimated H-index: 1
(Weyerhaeuser)
The combination of abscisic acid (ABA) and activated carbon increased Norway spruce (Picea abies L., Karst.) cotyledonary somatic embryo yields, increased the number of genotypes forming cotyledonary embryos, caused embryos to form that exhibited improved maturation characteristics, and reduced embryo production costs. Somatic embryos increased in size, showed larger apical regions, became more zygotic-like in shape, and showed higher percentages of epicotyl development upon germination. Analyse...
42 Citations Source Cite
Published on Sep 1, 2003in Plant Cell Reports 2.99
Gerald S. Pullman25
Estimated H-index: 25
,
Y. Zhang2
Estimated H-index: 2
,
B. H. Phan1
Estimated H-index: 1
(University of Georgia)
Somatic embryogenesis (SE), the most promising technology for the large-scale production of high-value coniferous trees from advanced breeding and genetic engineering programs, is expected to play an important role in increasing productivity, sustainability, and the uniformity of future U.S. forests. To be successful for commercial use, SE technology must work with a variety of genetically diverse trees. Initiation in loblolly pine (Pinus taeda L.), our main focus species, is often recalcitrant ...
76 Citations Source Cite
Published on Mar 1, 2006in Plant Science 3.71
Gerald S. Pullman25
Estimated H-index: 25
(Georgia Institute of Technology),
R. Chopra1
Estimated H-index: 1
(Georgia Institute of Technology),
Kelly-Marie Chase2
Estimated H-index: 2
(Georgia Institute of Technology)
Abstract A major barrier to the commercialization of somatic embryogenesis technology in loblolly pine ( Pinus taeda L.) is recalcitrance of some high-value crosses to initiate embryogenic tissue and continue early-stage somatic embryo growth. Developing initiation and multiplication media that resemble the seed environment may decrease this recalcitrance. Organic acid analyses were performed weekly throughout the sequence of seed development for female gametophyte and zygotic embryo tissues to ...
42 Citations Source Cite
Published on Dec 1, 2003in Plant Cell Reports 2.99
S. C. Van Winkle2
Estimated H-index: 2
(International Paper),
Gerald S. Pullman25
Estimated H-index: 25
(Georgia Institute of Technology)
This study considered the elemental composition of plant tissue culture media in response to pH and two different types of activated C (AC; tissue culture and non acid-washed grades) in liquid media. When tissue culture medium is supplemented with AC the method of AC addition and pH adjustment can greatly impact the final medium pH, in turn, altering mineral availability. Over the pH range of 4–7, Cu and Zn adsorbed (95% and 50%) onto the two physically different ACs to the same extent. As the p...
28 Citations Source Cite
Published on Jan 1, 1999in Applied Biochemistry and Biotechnology 1.80
John Cairney59
Estimated H-index: 59
,
Nanfei Xu5
Estimated H-index: 5
+ 2 AuthorsBarbara Johns2
Estimated H-index: 2
In production biological systems, monitoring and controlling the growth environment is possible, but assessing the metabolic competency of the organism is more difficult. Somatic embryogenesis (SE), a tissue-culture method for multiplying embryos asexually, has great potential to capture at low cost the genetic gain from breeding and genetic-engineering programs. Loblolly pine, however, has proven recalcitrant in the production of somatic embryos suitable in quality for operational use. Many sim...
27 Citations Source Cite
  • References (34)
  • Citations (18)
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Cited By18
Published on Mar 1, 2016in Protoplasma 2.46
Neusa Steiner11
Estimated H-index: 11
(Universidade Federal de Santa Catarina),
Francine L. Farias-Soares5
Estimated H-index: 5
(Universidade Federal de Santa Catarina)
+ 7 AuthorsMiguel Pedro Guerra28
Estimated H-index: 28
(Universidade Federal de Santa Catarina)
Somatic embryogenesis is a morphogenetic route useful for the study of embryonic development, as well as the large-scale propagation of endangered species, such as the Brazilian pine (Araucaria angustifolia). In the present study, we investigated the morphological and ultrastructural organization of A. angustifolia somatic embryo development by means of optical and electron microscopy. The proembryogenic stage was characterized by the proliferation of proembryogenic masses (PEMs), which are cell...
12 Citations Source Cite
Published on Oct 1, 2015in Trees-structure and Function 1.78
Qixiang Zhang4
Estimated H-index: 4
,
Weibo Xiang + 7 AuthorsJianqin Huang4
Estimated H-index: 4
Key message This paper provides an important direct proembryogenic mass initiation and plant regeneration method that might be supportive and stable for gene transformation and commercial production of Torreya grandis. Abstract Torreya grandis is an important and commer- cially valuable tree that is utilized as a source of essential oil and nuts. Propagation researches of T. grandis have been focused on micropropagation and somatic embryogenesis which derived from callus but limited data regardi...
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Published on Jan 1, 2016in Journal of Proteomics 3.72
André Luis Wendt dos Santos8
Estimated H-index: 8
(University of São Paulo),
Paula Elbl4
Estimated H-index: 4
(University of São Paulo)
+ 4 AuthorsEny Iochevet Segal Floh24
Estimated H-index: 24
(University of São Paulo)
GeLC–MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angustifolia RNA-Seq data, 2398 non-redundant proteins were identified. The log2 of the spectral count values of 858 proteins of these proteins showed a normal distribution, an...
13 Citations Source Cite
Published on May 1, 2013in Acta Physiologiae Plantarum 1.44
Sharrmila Rengeswari Palanyandy3
Estimated H-index: 3
(Universiti Putra Malaysia),
Periasamy Suranthran5
Estimated H-index: 5
(Universiti Putra Malaysia)
+ 5 AuthorsSiti Habsah Roowi3
Estimated H-index: 3
In the present study, we report the in vitro development of polyembryoids with identification of a definite stage that can be used for subsequent uniform plantlet regeneration in oil palm (Elaeis guineensis Jacq.). Induction and maturation of polyembryoids was accomplished when cell suspension culture was transferred in MS (Murashige and Skoog, Physiol Plant 15:473–497, 1962) semisolid medium consisting of 30 g L−1 sucrose and 3.5 g L−1 gelrite® devoid of any plant growth regulator. Growth and d...
9 Citations Source Cite
Published on May 1, 2014in New Forests 2.66
Gerald S. Pullman25
Estimated H-index: 25
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
The shift from vegetative to embryogenic growth requires tissue to enter a radically different program of development and can be studied in vitro through the development of somatic embryos. From an applied perspective somatic embryogenesis (SE) is expected to play an important role in increasing productivity, sustainability, and uniformity of future forests. For commercial use, SE technology must work with a variety of genetically diverse trees. Since the first reports of SE in Picea abies and L...
21 Citations Source Cite
Published on Jan 1, 2014in Tree Physiology 3.39
Leonardo Jo3
Estimated H-index: 3
(University of São Paulo),
André Luis Wendt dos Santos8
Estimated H-index: 8
(University of São Paulo)
+ 2 AuthorsEny Iochevet Segal Floh24
Estimated H-index: 24
(University of São Paulo)
Somatic embryogenesis is an important biotechnological tool in the large-scale propagation of elite genotypes and ex situ conservation of conifer species. Protocols for the induction and proliferation of embryogenic cultures (ECs) of Brazilian pine (Araucaria angustifolia (Bert.) O. Ktze) are well established, although the proper formation of mature somatic embryos (SEs) is still problematic. Thus, the identification of molecular markers for the screening of ECs able to respond to maturation con...
26 Citations Source Cite
Published on May 1, 2016in New Forests 2.66
Gerald S. Pullman25
Estimated H-index: 25
(Georgia Institute of Technology),
Katie Olson1
Estimated H-index: 1
(Georgia Institute of Technology)
+ 3 AuthorsKylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
Fraser fir (Abies fraseri [Pursh] Poir.) is a coniferous species native to the Southern Appalachian Mountains of the eastern United States. The species has high economic and recreational value but is vulnerable to extinction due to introduced pests and global warming. Somatic embryogenesis technology may assist in the clonal production of desired lines of Christmas trees and safekeeping of rare and valuable germplasm via cryopreservation. We have developed a highly effective medium for initiatio...
10 Citations Source Cite
Published on Jan 1, 2016in Methods of Molecular Biology
Krystyna Klimaszewska20
Estimated H-index: 20
,
Catherine Hargreaves2
Estimated H-index: 2
(Scion)
+ 1 AuthorsJean-François Trontin13
Estimated H-index: 13
This review compiles research results published over the last 14 years on conifer somatic embryogenesis (SE). Emphasis is placed on the newest findings that affect the response of seed embryos (typical explants) and shoot primordia (rare explants) to the induction of SE and long-term culture of early somatic embryos. Much research in recent years has focused on maturation of somatic embryos, with respect to both yield and quality, as an important stage for the production of a large number of vig...
31 Citations Source Cite
Published on Oct 1, 2017in Industrial Crops and Products 3.85
Sandra Nunes3
Estimated H-index: 3
,
Liliana Marum7
Estimated H-index: 7
+ 4 AuthorsConceição Santos32
Estimated H-index: 32
(University of Porto)
Abstract Clonal breeding programs of the highly important interspecific hybrid Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis require the establishment of robust cryopreservation protocols, with no genetic variability. Embryonal masses of this hybrid were successfully cryopreserved by a fast slow-freezing method. The protocol was optimized by testing the effects of dimethyl sulfoxide (DMSO) based cryoprotectant solutions, sugar cryoprotectants (e.g. sucrose or maltose, 0.4 M)...
6 Citations Source Cite
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