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Torreya taxifolia Arn., an ancient evergreen tree, is on the brink of extinction from attack by a fungal disease, recently reported to be caused by a novel isolate of Fusarium. We report the development of a somatic embryogenesis tissue culture system that can be used for cryogenic storage of T. taxifolia cultures and subsequent plant regeneration. Initiation of embryogenic tissue from immature zygotic embryos occurred on a conifer tissue culture medium containing 0.25 % activated charcoal, 43.8 mM maltose, 0.5 mM 2,4-dichlorophenoxacetic acid, 0.2 mM 6-benzylaminopurine, 0.2 mM kinetin, 0.1 μM brassinolide, 3.8 μM abscisic acid, 20.5 μM biotin, 1.13 μM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Embryo induction ranged from 60 to 100 % across six seed sources. Somatic embryo development occurred on a medium containing 43.8 mM maltose, 1 % activated charcoal, 37.8 μM abscisic acid, 20.5 μM biotin, 0.1 μM brassinolide, 0.205 mM folic acid, 1.28 mM 2(n-morpholino)ethanesulfonic acid and 0.69 mM pyruvic acid. Germination of somatic embryos ranged from 64 to 82 %. Embryogenic tissue cultures from 30 genotypes representing seed from six mother trees were cryopreserved, and culture recovery was demonstrated after freezing. In contrast to many other coniferous tree seeds, the measured water potential (−MPa) of T. taxifolia megagametophyte tissue rose greatly during seed after-ripening. Duplication of this rise in vitro allowed development of somatic embryos to the cotyledonary stage.
Recent advances in conifer somatic embryogenesis: improving somatic embryo quality
Claudio Stasolla22
Estimated H-index: 22
(University of Winnipeg),
Edward C. Yeung36
Estimated H-index: 36
(University of Calgary)
Somatic embryogenesis of coniferous species was first reported more than 20 years ago. Since then, there has been an explosion of research aimed at developing and optimizing protocols for efficient regeneration of plantlets. Although routinely used both as a means of propagation, as well as a valuable model system for investigating the structural, physiological, and molecular events occurring duri...
Cited 177 Source Cite this paper Add Collection
Rapid and Reliable Differential Display from Minute Amounts of Tissue: Mass Cloning and Characterization of Differentially Expressed Genes from Loblolly Pine Embryos
Nanfei Xu6
Estimated H-index: 6
Barbara Johns3
Estimated H-index: 3
Gerald S. Pullman17
Estimated H-index: 17
... (1 others)
Performing RNA differential display analysis on small tissue samples is difficult since much RNA, the initial template for the reaction, is lost during conventional isolation procedures. We have developed a rapid method which employs oligo-dT beads to capture mRNA from cell lysates. Subsequent reactions are primed directly from the beads, thus RT and PCR reactions can be completed within a few hou...
Ref 15Cited 23 Source Cite this paper Add Collection
A Handbook of the World's ConifersForeword Preface The conifers of the world, an introduction The distribution and ecology of conifers The economic importance of conifers The conservation of conifer diversity Synopsis of families and genera Taxonomic treatment of families, with keys to families Taxonomic treatment of genera and species (in alphabetical order), with keys to sections, subsections and species Glossary References List...
Cited 97 Source Add Collection
"Improving loblolly pine somatic embryo maturation: comparison of somatic and zygotic embryo morphology, germination, and gene expression"
2003 in Plant Cell Reports [IF: 2.87]
Gerald S. Pullman18
Estimated H-index: 18
(Georgia Institute of Technology),
Shannon Johnson9
Estimated H-index: 9
(Georgia Institute of Technology),
Gary F. Peter23
Estimated H-index: 23
(University of Florida)
... (2 others)
Clonal production of loblolly pine (Pinus taeda L.) through somatic embryogenesis has the potential to meet the increasing industrial demands for high-quality uniform raw materials. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Twenty-five newly initiated loblolly pine genotypes were followed through the process of liquid culture establish...
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Natural and Somatic Embryo Development in Loblolly PineIn production biological systems, monitoring and controlling the growth environment is possible, but assessing the metabolic competency of the organism is more difficult. Somatic embryogenesis (SE), a tissue-culture method for multiplying embryos asexually, has great potential to capture at low cost the genetic gain from breeding and genetic-engineering programs. Loblolly pine, however, has proven...
Ref 20Cited 16 Download Pdf Cite this paper Add Collection
Plant regeneration from somatic embryos ofTaxus brevifolia
1996 in Plant Cell Reports [IF: 2.87]
Paula P. Chee1
Estimated H-index: 1
Taxusbrevifolia is the source of paclitaxel (Taxol®), an anticancer drug. A method for regeneration ofTaxus brevifolia from immature zygotic embryos via somatic embryogenesis is described. Embryogenic callus tissues were obtained by culturing immature zygotic embryos on Lloyd and McCown medium (MCM) supplemented with 160 μM 2,4-dichlorophenoxyacetic acid (2,4-D) + 5 μM benzylaminopurine (BA) + 5 μ...
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Improved Norway spruce somatic embryo development through the use of abscisic acid combined with activated carbon
2005 in Plant Cell Reports [IF: 2.87]
Gerald S. Pullman17
Estimated H-index: 17
Pramod K. Gupta11
Estimated H-index: 11
Roger Timmis11
Estimated H-index: 11
... (3 others)
The combination of abscisic acid (ABA) and activated carbon increased Norway spruce (Picea abies L., Karst.) cotyledonary somatic embryo yields, increased the number of genotypes forming cotyledonary embryos, caused embryos to form that exhibited improved maturation characteristics, and reduced embryo production costs. Somatic embryos increased in size, showed larger apical regions, became more zy...
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Brassinolide improves embryogenic tissue initiation in conifers and rice
2003 in Plant Cell Reports [IF: 2.87]
G. S. Pullman1
Estimated H-index: 1
Y. Zhang1
Estimated H-index: 1
Bao H. Phan4
Estimated H-index: 4
(University of Georgia)
Somatic embryogenesis (SE), the most promising technology for the large-scale production of high-value coniferous trees from advanced breeding and genetic engineering programs, is expected to play an important role in increasing productivity, sustainability, and the uniformity of future U.S. forests. To be successful for commercial use, SE technology must work with a variety of genetically diverse...
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Cited by17
Quantitative proteomic analysis of Araucaria angustifolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential
2016 in Journal of Proteomics [IF: 3.91]
André Luis Wendt dos Santos5
Estimated H-index: 5
(University of São Paulo),
Paula Elbl4
Estimated H-index: 4
(University of São Paulo),
Bruno Viana Navarro2
Estimated H-index: 2
(University of São Paulo)
... (4 others)
GeLC–MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angustifolia RNA-Seq data, 2398 non-redundant proteins were identified. The ...
Ref 100Cited 8 Source Cite this paper Add Collection
Pine somatic embryogenesis: analyses of seed tissue and medium to improve protocol development
2014 in New Forests [IF: 1.54]
Gerald S. Pullman18
Estimated H-index: 18
(Georgia Institute of Technology),
Kylie Bucalo7
Estimated H-index: 7
(Georgia Institute of Technology)
The shift from vegetative to embryogenic growth requires tissue to enter a radically different program of development and can be studied in vitro through the development of somatic embryos. From an applied perspective somatic embryogenesis (SE) is expected to play an important role in increasing productivity, sustainability, and uniformity of future forests. For commercial use, SE technology must ...
Cited 18 Download Pdf Cite this paper Add Collection
Cryopreservation of the critically endangered golden paintbrush (Castilleja levisecta Greenm.): from nature to cryobank to nature
Ahmed Salama (University of Guelph), Elena Popova5
Estimated H-index: 5
(University of Guelph),
Maxwell P. Jones2
Estimated H-index: 2
(University of Guelph)
... (3 others)
In this study conservation of Castilleja levisecta Greenm., a globally endangered species was addressed through in vitro cryopreservation of shoot tips. In vitro cultures were successfully established using seedlings received from British Columbia, Canada. Shoot tips excised from in vitro propagated plants were cryopreserved using a droplet-vitrification method following optimization of individual...
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Direct proembryogenic masses initiation and plant regeneration from immature Torreya grandis embryos
Qixiang Zhang1
Estimated H-index: 1
(Agriculture and Forestry University),
Weibo Xiang (Agriculture and Forestry University), Jin Yao (Agriculture and Forestry University) ... (6 others)
Key message This paper provides an important direct proembryogenic mass initiation and plant regeneration method that might be supportive and stable for gene transformation and commercial production of Torreya grandis. Abstract Torreya grandis is an important and commer- cially valuable tree that is utilized as a source of essential oil and nuts. Propagation researches of T. grandis have been focu...
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In vitro developmental study of oil palm (Elaeis guineensis Jacq.) polyembryoids from cell suspension using scanning electron microscopy
2013 in Acta Physiologiae Plantarum [IF: 1.36]
Sharrmila Rengeswari Palanyandy3
Estimated H-index: 3
(Universiti Putra Malaysia),
Periasamy Suranthran5
Estimated H-index: 5
(Universiti Putra Malaysia),
Saikat Gantait8
Estimated H-index: 8
(Universiti Putra Malaysia)
... (5 others)
In the present study, we report the in vitro development of polyembryoids with identification of a definite stage that can be used for subsequent uniform plantlet regeneration in oil palm (Elaeis guineensis Jacq.). Induction and maturation of polyembryoids was accomplished when cell suspension culture was transferred in MS (Murashige and Skoog, Physiol Plant 15:473–497, 1962) semisolid medium cons...
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