Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2

Significance Eukaryotic genes are directed to distinct subnuclear compartments to regulate their activity. We show that different regions of the murine T-cell receptor β ( Tcrb ) locus interact independently with the nuclear lamina and that these interactions locally suppress the recombination of variable, diversity, and joining gene segments. This suppression is associated with the physical segregation of the locus from the recombinase protein,...