In situX-ray crystallography

A method is proposed, and preliminary experiments are described, for collection of X-ray data from macromolecular crystals in situ . The usual processes of mounting for either room-temperature or cryogenic X-ray data collection are eliminated by growing crystals, using vapor diffusion, on small supports or films that can be either frozen or treated before transfer directly to the X-ray beam. The approach has the advantage that individual...