Match!

High throughput methods of assessing protein stability and aggregation

Published on Jan 1, 2009in Molecular BioSystems2.85
· DOI :10.1039/B814377C
Guillermo Senisterra24
Estimated H-index: 24
(SGC: Structural Genomics Consortium),
Patrick J. Finerty16
Estimated H-index: 16
(SGC: Structural Genomics Consortium)
Abstract
The significant increase in the demand for purified protein for crystallization and structural studies has made necessary the development of multi-sample methods for identifying solution conditions that affect protein stability and aggregation. Conditions that stabilize proteins can improve protein purification and crystallization. These methods can be used to identify small molecule compounds or inhibitors that interact with the purified proteins, and might serve as starting points for drug discovery. In this article three methods for measuring protein stability and aggregation are described and discussed: differential scanning fluorimetry (DSF), differential static light scattering (DSLS), and isothermal denaturation (ITD).
  • References (37)
  • Citations (80)
References37
Newest
#1Guillermo Senisterra (SGC: Structural Genomics Consortium)H-Index: 24
#2Bum Soo Hong (SGC: Structural Genomics Consortium)H-Index: 8
Last.Masoud Vedadi (SGC: Structural Genomics Consortium)H-Index: 46
view all 4 authors...
#1Alexander I. Alexandrov (Scripps Research Institute)H-Index: 2
#2Mauro Mileni (Scripps Research Institute)H-Index: 14
Last.Raymond C. Stevens (Scripps Research Institute)H-Index: 95
view all 5 authors...
#1Oleg Fedorov (SGC: Structural Genomics Consortium)H-Index: 39
#2Brian D. Marsden (SGC: Structural Genomics Consortium)H-Index: 27
Last.Stefan Knapp (SGC: Structural Genomics Consortium)H-Index: 76
view all 9 authors...
#1Frank H. Niesen (SGC: Structural Genomics Consortium)H-Index: 18
#2Helena Berglund (SGC: Structural Genomics Consortium)H-Index: 22
Last.Masoud Vedadi (SGC: Structural Genomics Consortium)H-Index: 46
view all 3 authors...
#1Abdellah Allali-Hassani (SGC: Structural Genomics Consortium)H-Index: 32
#2Patricia W. Pan (SGC: Structural Genomics Consortium)H-Index: 8
Last.C.H. Arrowsmith (U of T: University of Toronto)H-Index: 74
view all 14 authors...
#1Guillermo Senisterra (SGC: Structural Genomics Consortium)H-Index: 24
#2Eugene MarkinH-Index: 1
Last.Donald E. Awrey (The Breast Cancer Research Foundation)H-Index: 11
view all 6 authors...
#1Ulrika B. Ericsson (Stockholm University)H-Index: 4
#2B. Martin Hallberg (Stockholm University)H-Index: 23
Last.Pär Nordlund (Stockholm University)H-Index: 46
view all 5 authors...
#1Andrew P. Yeh (CU: University of Colorado Boulder)H-Index: 1
#2Andy Mcmillan (CU: University of Colorado Boulder)H-Index: 1
Last.Michael H. B. Stowell (CU: University of Colorado Boulder)H-Index: 19
view all 3 authors...
Cited By80
Newest
#1Anders O. Magnusson (Technische Universität Darmstadt)H-Index: 1
#2Anna Szekrenyi (Technische Universität Darmstadt)H-Index: 5
Last.Wolf-Dieter Fessner (Technische Universität Darmstadt)H-Index: 32
view all 6 authors...
#1Shreya Dharadhar (NKI-AVL: Netherlands Cancer Institute)H-Index: 2
#2Robbert Q. Kim (NKI-AVL: Netherlands Cancer Institute)H-Index: 2
Last.Titia K. Sixma (NKI-AVL: Netherlands Cancer Institute)
view all 4 authors...
#1Tânia G. Lucas (University of Lisbon)H-Index: 4
#2Tânia G. Lucas (University of Lisbon)H-Index: 1
Last.Bárbara J. Henriques (University of Lisbon)H-Index: 1
view all 3 authors...
#1Jacob Blaffert (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 1
#2Haleh H. Haeri (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 3
Last.Patrick Garidel (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 40
view all 5 authors...
#1Mir Hussain Nawaz (NYUAD: New York University Abu Dhabi)H-Index: 2
#2Juliana C. Ferreira (NYUAD: New York University Abu Dhabi)
Last.Wael M. Rabeh (NYUAD: New York University Abu Dhabi)H-Index: 12
view all 7 authors...
#1Ren-Bin Zhou (NPU: Northwestern Polytechnical University)H-Index: 5
#2Xiao-Li Lu (NPU: Northwestern Polytechnical University)H-Index: 2
Last.Da-Chuan Yin (NPU: Northwestern Polytechnical University)H-Index: 16
view all 6 authors...
#1Tomohiko Matsuzawa (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 11
#2Masahiro Watanabe (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 66
Last.Katsuro Yaoi (AIST: National Institute of Advanced Industrial Science and Technology)H-Index: 20
view all 3 authors...
View next paperHigh-density miniaturized thermal shift assays as a general strategy for drug discovery.