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Cell-free protein synthesis: Applications come of age

Published on Sep 1, 2012in Biomedicine & Pharmacotherapy3.74
· DOI :10.1016/j.biotechadv.2011.09.016
Erik D. Carlson4
Estimated H-index: 4
(NU: Northwestern University),
Rui Gan5
Estimated H-index: 5
(NU: Northwestern University)
+ 1 AuthorsMichael Christopher Jewett36
Estimated H-index: 36
(NU: Northwestern University)
Abstract
Abstract Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and reaction scale has reached the 100-liter milestone. These advances have inspired new applications in the synthesis of protein libraries for functional genomics and structural biology, the production of personalized medicines, and the expression of virus-like particles, among others. In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications.
  • References (99)
  • Citations (318)
References99
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#1Yoshihiro Sasaki (Tokyo Medical and Dental University)H-Index: 22
#2Wakiko Asayama (Tokyo Medical and Dental University)H-Index: 3
Last.Kazunari Akiyoshi (Kyoto University)H-Index: 49
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#1Ho-Cheol Kim (CNU: Chungnam National University)H-Index: 7
#2Tae-Wan Kim (CNU: Chungnam National University)H-Index: 11
Last.Dong-Myung Kim (CNU: Chungnam National University)H-Index: 36
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#1Jerome Chalmeau (University of Toulouse)H-Index: 4
#2Nadezda Monina (UMN: University of Minnesota)H-Index: 1
Last.Vincent Noireaux (UMN: University of Minnesota)H-Index: 27
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#1Ruba Khnouf (UF: University of Florida)H-Index: 7
#2Daniel Olivero (UF: University of Florida)H-Index: 3
Last.Z. Hugh Fan (UF: University of Florida)H-Index: 24
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Cited By318
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#1Aidan Tinafar (U of T: University of Toronto)
#2Katariina Jaenes (U of T: University of Toronto)
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#1Zhi-Cong Liang (SCUT: South China University of Technology)
#2Ming-Hua Liang (SCUT: South China University of Technology)H-Index: 2
Last.Jian-Guo Jiang (SCUT: South China University of Technology)H-Index: 2
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#1Paul O. Sheridan (UCC: University College Cork)H-Index: 5
#2Jennifer C. Martin (Aberd.: University of Aberdeen)H-Index: 30
Last.Karen P. Scott (Aberd.: University of Aberdeen)H-Index: 42
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#1Adam D. Silverman (NU: Northwestern University)H-Index: 2
#2Umut Akova (NU: Northwestern University)
Last.Julius B. Lucks (NU: Northwestern University)H-Index: 26
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