Rui Gan
Northwestern University
Publications 5
#1Arturo Casini (Broad Institute)H-Index: 5
#2Fang Yuan Chang (MIT: Massachusetts Institute of Technology)H-Index: 2
Last.D. Benjamin Gordon (MIT: Massachusetts Institute of Technology)H-Index: 20
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Centralized facilities for genetic engineering, or “biofoundries”, offer the potential to design organisms to address emerging needs in medicine, agriculture, industry, and defense. The field has seen rapid advances in technology, but it is difficult to gauge current capabilities or identify gaps across projects. To this end, our foundry was assessed via a timed “pressure test”, in which 3 months were given to build organisms to produce 10 molecules unknown to us in advance. By applying a divers...
28 CitationsSource
The ability to site-specifically incorporate non-canonical amino acids (ncAAs) into proteins has made possible the study of protein structure and function in fundamentally new ways, as well as the bio synthesis of unnatural polymers. However, the task of site-specifically incorporating multiple ncAAs into proteins with high purity and yield continues to present a challenge. At the heart of this challenge lies the lower efficiency of engineered orthogonal translation system components compared to...
13 CitationsSource
We report a novel in vitro yeast ribosome display method based on cell-free protein synthesis (CFPS) using linear DNA templates. We demonstrate that our platform can enrich a target gene from a model library by 100-fold per round of selection. We demonstrate the utility of our approach by evolving cap-independent translation initiation (CITI) sequences, which result in a 13-fold increase in CFPS yields after four rounds of selection, and a threefold further increase by placing the beneficial sho...
5 CitationsSource
#1Rui Gan (NU: Northwestern University)H-Index: 5
Cell-free protein synthesis (CFPS) provides a valuable platform for understanding, using, and expanding the capabilities of the translation apparatus. For example, high-throughput CFPS is helping to address the increasing discrepancy between genome sequence data and their translation products. Here, we report the development of a combined cell-free transcription-translation (Tx/Tl) system from Saccharomyces cerevisiae that is suitable for such efforts. First, we show the ability to enable transl...
38 CitationsSource
#1Erik D. Carlson (NU: Northwestern University)H-Index: 4
#2Rui Gan (NU: Northwestern University)H-Index: 5
Last.Michael Christopher Jewett (NU: Northwestern University)H-Index: 36
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Abstract Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the growing demand for simple and efficient protein production. While used for decades as a foundational research tool for understanding transcription and translation, recent advances have made possible cost-effective microscale to manufacturing scale synthesis of complex proteins. Protein yields exceed grams protein produced per liter reaction volume, batch reactions last for multiple hours, costs...
318 CitationsSource