Building a Better Engine: Stimulation of Single Molecules of SGS1 by RPA and Top3-RMI1

Sgs1 is the Bloom's Syndrome helicase (BLM) ortholog and the sole RecQ-family helicase in Saccharomyces cerevisae. Genetic defects in Sgs1 cause gross chromosomal rearrangements and in humans manifest as Bloom's Syndrome, a disease associated with accelerated onset of sporadic cancers. Early during homologous recombination-mediated repair of dsDNA breaks, Sgs1 stimulates long-range resection in coordination with Mre11/Rad50/Xrs2 and the...