Assessment of cholesterol requirements in the prawn, Penaeus japonicus

Two feeding experiments were conducted to assess the quantitative cholesterol requirement of the juvenile prawn, Penaeus japonicus. The prawns were fed casein-based (experiment 1) and casein- or crab protein-based (experiment 2) diets with or without supplemental cholesterol. The daily increases in quantities of body cholesterol (mg kg−1 prawn day−1) at maximum growth and dietary cholesterol intake over a 40-day feeding period were determined....