Optically Modulated Fluorescent Proteins Enhance Sensitivity in Live Cell Imaging

Fluorescence microscopy is a widely used non-invasive tool for investigating intracellular structure and processes. Although the palette of fluorescent proteins has revolutionized detection and dynamics in molecular and cellular biology, the limited brightness, low spectral discrimination, and high cellular autofluorescence continue to limit applications. In contrast to photoswitch-based methods, we have developed a spectroscopic method to...