Catalytically Active MAP KAP Kinase 2 Structures in Complex with Staurosporine and ADP Reveal Differences with the Autoinhibited Enzyme

MAP KAP kinase 2 (MK2), a Ser/Thr kinase, plays a crucial role in the inflammatory process. We have determined the crystal structures of a catalytically active C-terminal deletion form of human MK2, residues 41–364, in complex with staurosporine at 2.7 Å and with ADP at 3.2 Å, revealing overall structural similarity with other Ser/Thr kinases. Kinetic analysis reveals that the Km for ATP is very similar for MK2 41–364 and p38-activated MK2...