Reproducibility of Complement 4d deposition by immunofluorescence and immunohistochemistry in lung allograft biopsies

The significance of Complement 4d (C4d) deposition in the diagnosis of antibody-mediated rejection (AMR) in lung allografts is controversial. A potential cause may be the problematic reproducibility. We studied the reproducibility of C4d by immunofluorescence (IF) and immunohistochemistry (IHC) in lung allograft transbronchial biopsies (TBBx), correlated C4d by IF with IHC, and compared the results with clinical findings.TBBx obtained between...