Preparation of Double Liposomes and Their Efficiency as an Oral Vaccine Carrier

The usefulness of double liposomes (DL), liposomes containing liposomes inside, as an oral vaccine carrier was examined. Ovalbumin (OVA) encapsulating liposomes sized to 230 nm (small liposomes, SL) were prepared by the glass-beads (GB) method and sequential sonication and extrusion. For the purpose of stabilizing the model antigen, DL containing SL were prepared by the GB method and the reverse-phase evaporation (REV) method. They were named...