Processing strategies and software solutions for data‐independent acquisition in mass spectrometry

Data‐independent acquisition (DIA) offers several advantages over data‐dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC‐MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation of all detectable ions within a wide m/z range regardless of intensity, thereby providing broader dynamic range of detected...